Abstract
The homologous recombination (HR) repair pathway maintains genetic integrity after DNA double-strand break (DSB) damage and is particularly crucial for maintaining fidelity of expressed genes. Histone H4 acetylation on lysine 16 (H4K16ac) is associated with transcription, but how pre-existing H4K16ac directly affects DSB repair is not known. To answer this question, we used CRISPR/Cas9 technology to introduce I-SceI sites, or repair pathway reporter cassettes, at defined locations within gene-rich (high H4K16ac/euchromatin) and gene-poor (low H4K16ac/heterochromatin) regions. The frequency of DSB repair by HR is higher in gene-rich regions. Interestingly, artificially targeting H4K16ac at specific locations using gRNA/dCas9-MOF increases HR frequency in euchromatin. Finally, inhibition/depletion of RNA polymerase II or Cockayne syndrome B protein leads to decreased recruitment of HR factors at DSBs. These results indicate that the pre-existing H4K16ac status at specific locations directly influences the repair of local DNA breaks, favoring HR in part through the transcription machinery.
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Introduction
In order to maintain genomic stability in gene-rich regions, especially where transcription occurs consistently, high-fidelity DNA repair by HR is required to avoid deleterious mutations. Whether such high-fidelity DNA repair is dependent upon specific chromatin modifications is under studied due to technical reasons. The chromatin modification of histone H4 by acetylation at K16, H4K16ac1, carried out by the MOF (males absent on the first) acetyltransferase2,3, is associated with transcription4,5,6 and has been shown to alter chromatin structure7. We previously reported that depletion of MOF decreased H4K16ac and overall DNA DSB repair levels2, but how the modification impacts DSB repair and fidelity in structurally or functionally distinct regions, is not known. The restoration of DNA fidelity post-DSB induction is ensured by homology directed repair, specifically the HR pathway. Transcriptionally active regions reportedly recruit factors involved in HR-mediated DSB repair8, consistent with recent studies identifying DSB-induced histone modifications involved in preferential repair by HR9 and human C-NHEJ proteins10,11,12. A major impediment in the mammalian DNA repair field has been the random nature of ionizing radiation (IR) or chemical-induced DNA damage, making it impossible to characterize how DNA DSB repair is influenced by chromatin context e.g., regions of coding (euchromatin/gene-rich/H4K16ac-rich) or intergenic noncoding (heterochromatin/gene-poor/H4K16ac-poor) regions13. We have circumvented this problem by using two different approaches: (1) using CRISPR/Cas9-based technology to introduce I-SceI cleavage sites and DSB repair cassettes at defined chromosomal sequences to measure DNA DSB repair14,15, and; (2) direct induction of a DNA DSB at defined sites in specific phases of cell cycle by using liposomes loaded with CRISPR Single-guide RNA (gRNA)15. Using these technological approaches, we report that DSB repair by the HR pathway takes place preferentially at higher frequency where H4K16ac is elevated in gene-rich transcribing regions during the S-phase of the cell cycle.
Results
Repair cassette insertion has minimum impact on histone status
To test whether preexisting H4K16ac levels regulate the frequency of DNA DSB repair by HR, specific sites on three human chromosomes (1, 5, and 17) were chosen for the study (Supplementary Fig. 1)13. Based on the relative location of genes and long intergenic regions, we selected three sites on chromosome 1 (Chr1A, Chr1B, and Chr1C); three sites on chromosome 5 (Chr5A, Chr5B, and Chr5C); and two sites on chromosome 17 (Chr17A and Chr17B) (Supplementary Fig. 1) showing similar levels of histone H4 but different levels of H4K16ac (Fig. 1a, b) in order to analyze the impact of chromatin status on DNA DSB repair. To measure HR, a DR-GFP cassette16 was inserted in cells at Chr1A, Chr1B, Chr1C, Chr5A, Chr5B, Chr5C, Chr17A, and Chr17B sites (Fig. 1c, Supplementary Table 1). The EJ5-GFP test cassette was inserted into cells at Chr1A and Chr1B sites17 to measure NHEJ mediated repair (Fig. 1c, Supplementary Table 1). The insertion sites at Chr1A, Chr1C, Chr5A, Chr5C, and Chr17A are between coding genes, whereas the insertion sites at Chr1B, Chr5B, and Chr17B are within the long noncoding intergenic regions and Chr17B is between KIF2B and TOM1L1 (Supplementary Fig. 2). In parallel, a single I-SceI endonuclease recognition sequence element without reporter cassette was inserted in another set of cell lines at the Chr1A, Chr1B, Chr5A, Chr5B, Chr17A, and Chr17B sites (Supplementary Table 1, Supplementary Fig. 2).
We determined histone H4 levels at the Chr1A, Chr1B, Chr5A, Chr5B, Chr17A, and Chr17B sites before and after DSB induction and did not detect any significant changes in histone H4, even after gRNA tethering of dCas9-hMOF at the sites (Fig. 1a). We subsequently determined H4K16ac levels before and after DR-GFP insertion at the defined regions and found that the relative abundance was unaltered, but observed amongst the sites gene-rich regions generally contained higher H4K16ac levels (Fig. 1d). Overall H4K16ac levels peak in S and G2/M phase cells, while hMOF depletion reduced H4K16ac in all phases of the cell cycle (Fig. 1e, Supplementary Fig. 3).
Impact of DSB induction on histone status
Induction of DSBs did not significantly alter H4K16ac levels in gene-rich regions at the sites examined (Fig. 2a). The efficiency of I-SceI induced DNA DSBs was identical between sites in gene-rich/H4K16ac rich (Chr1A) and gene-poor/H4K16ac poor (Chr1B) (Fig. 2b). DSB levels were increased at both sites after KU80 depletion (Fig. 2b). The DNA cleavage was confirmed by detection of γ-H2AX or 53BP1 foci in cells 15 h post transfection with I-SceI expression vector (Fig. 2c). Alternatively, an I-SceI nuclear translocation system induced DNA breaks within 15 min of drug treatment18, with similar cutting efficiencies obtained throughout the cell cycle (Fig. 2d–e). Similar DNA break kinetics were also obtained using a newly developed CRISPR gRNA liposomes to induce the DSB (Fig. 2f–g, Supplementary Fig. 4). All three approaches indicated that, among the selected sites, no significant difference was observed in DNA DSB induction between gene-rich and gene-poor regions (Fig. 2a–g, Supplementary Fig. 4).
Insertion of cassettes has minimum impact on DNA damage response
Relative frequency of DNA DSB repair by NHEJ and HR functions were measured in cells by repair-dependent reconstitution of disrupted GFP repair cassettes after DSB induction by ectopic I-SceI endonuclease expression19,20. First, we determined whether the insertion of I-SceI sequences, DR-GFP or EJ5-GFP cassettes (Fig. 1c,d, Supplementary Fig. 2) impacts the global DNA damage response. Clonogenic survival, repairosome formation, and residual chromosome aberrations after IR exposure were all unaltered in the different cell lines (Fig. 3). This suggests that introduction of I-SceI elements or DNA repair reporter cassettes did not impact global DNA damage sensing, response, and repair.
H4K16ac levels impact DSB repair by HR in gene-rich regions
Since there was no difference in DNA DSB induction between different targeted sites, we next examined whether the repair pathway at the different sites was influenced by the context of a high or low H4K16ac density. We first compared DSB repair by NHEJ at different insertion sites with and without ligase IV depletion and found DSB repair efficiency to be similar irrespective of the H4K16ac status (Supplementary Fig. 5).
We next examined whether chromatin status impacts HR-mediated DSB repair (DR-GFP inserted sites) (Supplementary Table 1, Supplementary Fig. 2) and found higher HR repair in gene-rich chromatin regions associated with higher H4K16ac levels as compared to the three different gene-poor chromosomal regions (Fig. 4a, Supplementary Fig. 6). Since there was a good correlation between H4K16ac and HR levels, we tested whether increasing local H4K16ac levels in gene-poor regions would increase HR repair frequency. A nuclease-dead dCas9-hMOF fusion protein (dCas9-hMOF) was ectopically expressed and tethered immediately upstream of the DR-GFP cassettes by coexpressing a guide RNA (gRNA) specific for each locus (Supplementary Fig. 7). Measurement by ChIP-qPCR of dCas9-hMOF tethering at the Chr1A, Chr1B, Chr5A, and Chr5B sites indicated successful localization, as judged by a significant increase in local H4K16ac levels (Fig. 4b, Supplementary Fig. 8), without affecting nucleosome occupancy/total H4 levels (Fig. 1a). The local H4K16ac level was not increased significantly by another acetyltransferase construct, dCas9-p300(Core), that does not affect H4K16ac status (Supplementary Fig. 8). The artificially increased local levels of H4K16ac (Fig. 4b) did not affect DNA DSB induction, as measured by LM-PCR analysis of I-SceI digestion efficiency (Supplementary Fig. 9). The HR efficiency at the Chr1A and Chr5A loci was increased significantly by dCas9-hMOF with gRNA, but no increase was detected at the Chr1B and Chr5B sites, indicating that artificially increased H4K16ac impacts HR efficiency only in euchromatic regions (Fig. 4c). Tethering of dCas9-p300 (Core) to DSB sites did not influence HR efficiency at any site (Fig. 4c).
The differences in HR repair frequency at gene-rich and gene-poor sites could be due to altered recruitment of HR-related factors. While there is no significant difference detected in phosphorylated H2AX (γ-H2AX) at the DSB sites, higher KU80 and 53BP1 signals are observed at Chr1B, whereas RAD51, BRCA1, and RPA2 levels are higher at Chr1A in exponentially growing cells (Fig. 4d–e). A cell cycle-regulated circuit, underpinned by RIF1 and BRCA1, governs DSB repair pathway choice to ensure that NHEJ dominates in G1 and HR is favored from S-phase onward21. We also found that in G1-arrested cells, KU80 and 53BP1 are increased at the Chr1B site, (Fig. 4f–g), whereas RAD51, BRCA1, and RPA2 levels are higher at Chr1A site in S-phase cells (Fig. 4h–i). Recruitment of repair proteins at Chr1A and Chr1B DSB sites in G2/M cells is similar to what is seen in G1 cells (Fig. 4j–k).
What favors the recruitment of HR proteins to DSB sites in high H4K16ac regions is not clear. SMARCAD1 protein is recruited to newly synthesized DNA and facilitates histone deacetylation, histone H3K9 trimethylation (H3K9me3), and efficient HP1 recruitment through a mechanism coupled to ATP hydrolysis22. SMARCAD1 is also recruited to DNA DSBs during DNA resection through an ATM-dependent process and has been suggested to assist nucleosome displacement during the resection process leading to HR19,23. Our analysis of SMARCAD1 recruitment to DSBs indicates enrichment is significantly higher at DSB sites located in gene-rich regions (Chr1A and Chr1C), compared to the Chr1B gene-poor region (Fig. 4l). A similar significant increase of SMARCAD1 levels is also seen at Chr17A after DSB induction with CRISPR liposomes at 120 min of treatment, compared to Chr17B (Supplementary Fig. 10).
RNA pol II and CSB loading at DSB sites is higher in gene-rich regions
Transcription is a critical factor in deciding which pathway is used for DSB repair24 as RNA polymerase II (RNAPII) recognizes DNA damage25. We observed that local RNAPII levels increase at the DSB site after break induction in gene-rich regions (Fig. 5a–b). While treatment of cells with drugs-blocking transcription (α-amanitin or actinomycin D) has little effect on DSB repair by NHEJ (Fig. 5c), repair by HR repair is significantly decreased at the Chr1A DSB site with no effect on Chr1B (Fig. 5d–e). Consistent with these results, α-amanitin treatment has no impact on KU80 or 53BP1 recruitment at DSB sites, whereas RAD51 and BRCA1 recruitment is significantly reduced at the Chr1A DSB site (Fig. 5f).
How HR-mediated DSB repair is influenced by transcription could relate to the CSB remodeler, which is an essential factor in transcription-coupled nucleotide excision repair (NER)24,26,27,28. We tested whether CSB interacts with RNAPII post DNA DSB induction and observed association in an ATM-dependent manner (Fig. 6a). Moreover, CSB levels increased after DSB induction only at DSB sites in the gene-rich regions (Fig. 6b–c). Interestingly, depletion of CSB (Fig. 6b) reduced H4K16ac levels after DSB induction (Fig. 6d), led to failure of RNAPII accumulation at the DSB sites (Fig. 6e), and decreased HR (Fig. 6f, Supplementary Fig. 11), without affecting repair by NHEJ (Fig. 6g). Thus, when cells are treated with α-amanitin, stalled RNAPII is reduced at DSBs (Fig. 6h), and there is reduced recruitment of HR-related factors (Fig. 5f) with failure of HR-mediated repair (Fig. 6e). These observations support the argument that RNAPII-mediated transcription is critical for DSB repair in gene-rich/H4K16ac rich regions. This is in agreement with a previous report that HR is linked to the H3K36me3 chromatin mark deposited on transcribed genes in an RNAPII-dependent manner29. Interestingly, hMOF is a part of the MSL complex, which contains a MSL3 subunit with a chromodomain specific for H3K36me32,30.
RNA pol II and CSB load on RPA2 gene DSB sites during repair
We consistently observed that HR frequency was higher in gene-rich/transcribed regions (euchromatin) as compared to nontranscribing regions. We tested whether different regions of a transcribing gene might be subject to preferential DSB repair. We selected the RPA2 gene for analysis and introduced I-SceI element (18 bp) at three different locations (Fig. 7a). Measured H4K16ac levels at the I-SceI sites were highest (i) in the promoter region and lowest (iii) at the end of RPA2 gene (Fig. 7b). All three DNA sites had similar I-SceI cleavage efficiencies with similar RPA2 protein levels (Supplementary Fig. 12). An increase in RNAPll and SMARCAD1 enrichment peaked around 45 min after DSB induction (Fig. 7c, d) with maximum levels and increased RNAPII association observed at the promoter DSB site (i) (Fig. 7e). CSB interacts with RNAPII and we found that CSB levels were also higher at the promoter site, increasing upon DSB induction (Fig. 7f). To determine whether accumulation of RNAPII is dependent on CSB, we depleted CSB and found decreased RNAPII/DNA signals, with no increase after DSB induction (Fig. 7g).
Consistent with the RNAPII results, SMARCAD1 enrichment after DSB induction was also maximal at the promoter region I-SceI site (Supplementary Fig. 13). When we examined repair proteins levels at the three different RPA2 gene sites, all had increased enrichment of HR-related factors after DSB induction, supporting the concept that the entire transcribed region is preferentially repaired by HR (Fig. 7h). Since H4K16ac status is coupled with transcriptional activation, we determined whether depletion of hMOF impacts H4K16ac levels in the RPA2 gene. As expected, MOF-specific knockdown reduced H4K16ac signal at all three sites along the RPA2 gene (Fig. 7b). Consistent with the reduced H4K16ac levels, RNAPII before and after DSB induction was also significantly reduced (Fig. 7i), as was CSB binding. (Fig. 7j). Reduction of H4K16ac had no effect on NHEJ protein recruitment, while recruitment of HR factors RAD51 and BRCA1 was significantly reduced after DSB induction (Fig. 7k). These results further indicate that transcriptionally active regions with higher H4K16ac levels preferentially repair DNA DSBs using the HR pathway, preserving the integrity of the most important sequences in the genome, the coding ones.
Discussion
The chromatin flanking a DNA DSB undergoes extensive post-translational modification, such as phosphorylation of H2AX31 at the initial stage and more other protein modifications during DSB repair32,33. In addition, histones are removed from around DSBs to enable DNA repair and, upon completion of repair, are restored to reestablish the initial chromatin structure32,34. The H4K16ac modification has been reported to modulate both higher order chromatin structure and functional interactions between a nonhistone protein and the chromatin fiber7. The histone H4K16ac mark is established by the MOF protein, which has also been shown to interact with a range of proteins that extend its potential significance well beyond transcription35. For example, MOF is an upstream regulator of the ATM (ataxia–telangiectasia mutated) protein, and MOF loss impacts ATM function that can result in an ataxia–telangiectasia (AT)-like neurological phenotype36,37. Conversely, ATM can also regulate MOF function through post-translational modifications38.
A major impediment in the mammalian DNA repair field to answering how DSB repair takes place within the context of chromatin status is the nonspecificity of DNA damage induced by agents, making it difficult to characterize how specific differences in chromatin environment impact recruitment of DNA lesion signaling/repair factors. We have circumvented this problem by using site-specific DSB induction in combination with a high-density genome wide map identifying H4K16ac-rich or poor chromosomal sites13. Utilizing this data, we directly tested the hypothesis that local chromosomal H4K16ac levels impact the recruitment of proteins involved in either major DSB repair pathway, as well as determined the frequency of HR in gene-rich and gene-poor regions. Thus we were able to show that genomic regions with elevated preexisting H4K16ac histone levels, which are linked with transcription, are associated with preferential recruitment of HR-related DSB repair proteins and an increased frequency of DSB repair by HR. Consistent with the role of H4K16ac in preferential repair in transcribed regions, we report that H4K16ac rich regions have higher levels of RNAPII and CSB whose inhibition/depletion reduces DSB repair by HR. MOF is the major enzyme acetylating histone H4 at K16, and its role in transcription and the DNA damage response is conserved among insects and mammals3,35,39,40. Active transcription and Rad52 recognition of associated R-loops selects a critical portion of DSBs for HR-mediated repair41. These sites may be further differentiated by a high density of preexisting H4K16ac marks since this chromatin modification also has a profound effect on the DNA structure7, as well as transcriptional functions6. While HR is thought to be the most efficient means for maintaining transcribed gene fidelity during DNA repair, a multi-invasion-induced rearrangement occurring during HR was recently identified which uniquely amplified the initial DNA damage and possibly increased genome rearrangements42. Long-read sequencing and improved mapping of repeats should enable better appreciation of the significance of HR-related recombination in generating genomic rearrangements. In summary, by using a combination of technologies and producing site-specific DSBs within a defined chromatin context, we have addressed a very important question that opens a new area of research into the DNA damage response and its interaction with the preexisting chromatin status.
Methods
Cell lines
H1299 (human nonsmall cell lung carcinoma cell line), HEK293 (Human embryonic kidney 293 cells), and U2OS (Human bone osteosarcoma epithelial cells) and HeLa cell lines (ATCC) were grown in DMEM (Sigma) and 10% fetal calf serum (Sigma) supplemented with 1% penicillin/streptomycin.
Clonogenic assay
Cells were irradiated with graded doses of X-rays as described previously19 and incubated for 10–14 days. Colonies were stained with 0.5% crystal violet (Gibco) in 20% methanol with 1% formaldehyde and counted. Each individual group was processed in triplicate and normalized to untreated controls. The survival graphs show combined data from at least three to four independent experiments, and bars show standard error.
Antibodies
Antibodies for histone H4 (Millipore, 05858), H4K16ac (Millipore, 07329) SMARCAD1 (Santa Cruz Biotech, sc-162233, Novus Bio NB100–79835), γ-H2AX (Millipore, JBW301), Ku80 (Cell Signaling, 2180P), RAD51 (Abcam, ab213), BRCA1 (Santa CruzBiotech,sc-642), 53BP1 (Santa Cruz Biotech, sc-22760), CSB (Santa Cruz, 25371, 166042), GAPDH (Protein Tech, HRP-6004), were used for western blotting, immunoprecipitation and immunofluorescence. Antibodies used for the chromatin immunoprecipitation (ChIP) assay were histone H4 (Millipore, 05858), H4K16ac (Millipore, 07329), SMARCAD1 (Abnova, H00056916-BO1P), γ-H2AX (Millipore, JBW301), 53BP1 (Santa Cruz Biotech, sc-22760), BRCA1 (Abcam), Ku80 (Thermo Fisher,MA5–12933), RAD51(Abcam,ab176458), RAD51 (Abcam, ab176458), and RNA polymerase II (Abcam, ab817).
Generation of CRISPR/Cas9-based cell line
gRNA against the sites described in Supplementary Figs. 1 and2, was designed by screening the target sequence with the online tool http://www.broadinstitute.org/rnai/public/analysis-tools/sgrna-design15. One high-score gRNA target sequence was detected and then targeted on the sense strand sequence (as shown in Supplementary Fig. 2). The gRNA module was generated by overlapping PCR protocol with minor modifications, and was subsequently cloned into the same pLX-sgRNA vector. Transfection of humanized Cas9 that contained lentiviral pCW-Cas9 and customized pLX-sgRNA plasmids into H1299 cells15, and cells were selected by Zeocin (505 µg/mL) and blusticidin (5 µg/mL).
gRNA generation by in vitro transcription
CRISPR gRNA was designed based on the sequence from43 and template oligonucleotides with reverse complement sequence including T7 promoter were ordered from Eurofins Genomics (Louisville, KY). Template oligonucleotides were transcribed into gRNA using the MEGAscript™ T7 Transcription Kit (Invitrogen, Carlsbad, CA) according to manufacturer’s protocol. gRNA was purified using Oligo Clean & ConcentratorTM (Zymo Research, Irvine, CA) according to manufacturer’s procedures. For concentration determination, produced gRNA was diluted 1:20 in nuclease-free water and measured using Take3 plates and Synergy H4 Hybrid Reader (Biotek, Winooski, VT).
gRNA sequences are shown in Table 1.
CRISPR gRNA liposomes design and characterization
Liposomes were prepared by lipid hydration-extrusion method. 3.62 mg soybean phosphatidylcholine (Lipoid S100, Lipoid, Germany), and 0.88 mg 1,2-dioleoyl-3-trimethylammonium-propane (chloride salt) (Avanti, Alabama, USA) were dissolved in 3 mL ethanol. To fluorescently label liposome, fluorescent phospholipid lissamine rhodamine B 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (rhodamine-DHPE, Invitrogen) was incorporated in the membrane at 2% of the total lipid to all liposome formulations.
Thin film was formed by evaporating the solvent for 30 min at 41 °C/ 150 rpm using rotary evaporator (Rotavapor, Buchi, Switzerland). The thin film was rehydrated with 0.5 mL PBS pH 7.2, followed by the sonication of the liposomes. Liposomes were sonicated intermittently for 15 min until the desired size was achieved using Branson 1510 (Branson, Danbury, CT).
CRISPR gRNA liposomes were prepared by adding 1 μg GeneArtTM PlatinumTM Cas9 nuclease (Thermo Scientific, Vilnius, Lithuania) to 400 ng gRNA in 50 μL serum free MEM medium. After incubation at room temperature for 5 min, 2 μL were added to liposomes were added to the Cas9-gRNA complex and incubated for 5 min prior to transfection. CRISPR gRNA liposomes’ size (164.4 nm, polydispersity index 0.15) and zeta potential (23.93 ± 1.48 mV) were assessed in triplicates by dynamic light scattering using Zetasizer instrument (Malvern, Worcestershire, UK).
Western blotting and Immunoprecipitation
Cell lysates preparation and western blotting were performed as described previously38. The cell extract immunoprecipitation procedure is the same as described previously36. Full blots corresponding to Figs. 1e and 6 are shown in Supplementary Figs. 14 and 15.
ChIP assay
Chromatin immunoprecipitation assays were carried out using the previously described standard procedures19,38,44. Cells were synchronized as described previously44, transfected with I-SceI endonuclease expression vector or treated with the drug or liposomes as described19,38,44. After protein–DNA crosslinking, the chromatin was centrifuged, the supernatant collected and diluted (1:10) with ChIP dilution buffer as described previously19. Diluted chromatin was incubated with specific antibody and Magna ChIP Protein A/Gbeads (Millipore) overnight at 4 °C, subsequent steps were performed to reverse the crosslinking and the DNA was purified using QIAquick Spin columns (Qiagen). qPCR was carried out with specific sets of primers at the proper melting temperatures. Each experiment was repeated 3–4 times with consistent results. The signal to input ratio was low but significantly higher in comparison to IgG control values. Similar low ratios have been reported by other investigators19,45 and, in this case, likely reflect technical limits on detection of a protein bound to a single DSB site (the single I-SceI site) in the entire genome of the cell. RPA2 primers around I-SceI sites are shown in Table 2.
DSB repair by NHEJ and HR assay
The DSB repair assay by NHEJ or HR was performed by the procedure described previously19. To perform the NHEJ assay, commercially available EJ5 GFP-Puro plasmid DNA was integrated at the different sites of chromosome 1. The HR assay was performed in H1299 cells with a stably integrated DR-GFP cassette at different sites as described previously19,46,47. The percentage of GFP-positive cells after I-SceI DSB induction was measured by flow cytometry and used to define NHEJ or HR repair.
The relative values of HR were determined by counting cells positive for GFP at the site of chromosome 1 A (Chr1A), which gave the maximum percentage of positive cells and this is considered as 1. In the HR assay, cells positive for GFP are counted at other sites (Chr1B, Chr1C, Chr5, and Chr17) and plotted relative to the value obtained for Chr1A. In the NHEJ assay, the maximum cells positive for GFP are observed at chromosome 1 site B (Chr1B) and relative values are plotted.
Chromosome aberrations
Chromosomal aberrations at metaphase were examined as previously described46. Fifty metaphases were scored for each experiment and each experiment was repeated three to four times.
Statistics
Data were expressed as mean ± SD from three to four different experiments, and were analyzed by two-tailed unpaired Student’s t test. Statistical significance was assessed at *p < 0.05, **p < 0.01, ***p < 0.01 and ****p < 0.0001.
Reporting summary
Further information on research design is available in the Nature Research Reporting Summary linked to this article.
Data availability
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Acknowledgements
We thank members of Pandita laboratory for discussions during the inception and the completion of this study, respectively. Thanks are due to Drs Albino Bacolla and Mauro Ferrari for their help. This study is supported the Houston Methodist Research Institute and NIH RO1 CA129537 and RO1 GM109768 to TKP.
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N.H., B.G., P.G.K., J.C., and T.K.P. designed research; N.H., R.K.P., F.L., V.K.C., S.H., N.T.H., P.G.K., S.C., and D.K.S., performed experiments, and N.H., B.G., J.C., C.R.H., and T.K.P. wrote paper.
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Horikoshi, N., Sharma, D., Leonard, F. et al. Pre-existing H4K16ac levels in euchromatin drive DNA repair by homologous recombination in S-phase. Commun Biol 2, 253 (2019). https://doi.org/10.1038/s42003-019-0498-z
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DOI: https://doi.org/10.1038/s42003-019-0498-z
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