Abstract
A series of steroidal[17,16-d]pyrimidines derived from dehydroepiandrosterone were designed and prepared by a convenient heterocyclization reaction. The in vitro anticancer activities for these obtained compounds were evaluated against human cancer cell lines (HepG2, Huh-7, and SGC-7901), which demonstrated that some of these heterocyclic pyrimidine derivatives exhibited significantly good cytotoxic activities against all tested cell lines compared with 5-fluorouracil (5-FU), especially, compound 3b exhibited high potential growth inhibitory activities against all tested cell lines with the IC50 values of 5.41 ± 1.34, 5.65 ± 1.02 and 10.64 ± 1.49 μM, respectively, which might be used as promising lead scaffold for discovery of novel anticancer agents.
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Introduction
With the constant increase in cancer mortality, cancer has gradually become one of the most complicated diseases that threaten public health of humans1. Although many effective therapeutic methods have been approved for cancer control, chemotherapy still remains a mainstay options for cancer treatment2,3. However, the emergence of side effects and multidrug-resistance have encouraged us to discover and identify novel synthetic or naturally occurring small molecules with highly effective bioactivity or therapeutic use. Therefore, searching for and developing new chemical entities with special characteristics as effective anticancer molecules are an important endeavor in the field of medicinal chemistry.
It is well known that pyrimidine core has wide occurrence in nature4, and which has also represent a typical class of heterocyclic scaffold in various compounds applied in the field of medicinal chemistry5,6,7,8,9,10,11,12,13,14,15, agrochemicals16,17,18,19,20,21,22,23,24, and materials25,26,27,28,29. Up to now, many pyrimidines analogues have been demonstrated to exhibit wide pharmacological activities mainly including anticancer, antiviral, antibacterial, antimalarial, antituberculosis activities. In particular, some compounds containing pyrimidine unit have been developed as highly effective anticancer or antibacterial drugs (Fig. 1), which further confirm pyrimidine ring is an important pharmacophore in the discovery of novel active molecules. Meanwhile, natural steroids, particularly dehydroepiandrosterone (DHEA) attract extensive interest of researchers, and many steroidal compounds bearing DHEA moiety have also emerged as highly potential pharmaceutical molecules due to their inherent bioactivity30,31,32. Very recently, a series of DHEA-dihydrazone derivatives have also been investigated by us33,34, and the results indicated some of the prepared molecules could inhibit the growth of human tumor cell lines, which also identify that this natural steroidal scaffold might contribute to the potential cytotoxic activity.
Due to the above description and our ongoing interest in the discovery of novel functional heterocycles, we wish to integrate the structural features of pyrimidines and DHEA scaffold to a core structure as shown in Fig. 2, and explore the potential antiproliferative effects for these novel molecules derived from natural steroids. So we focused on the convenient synthesis, biological evaluation, and structure-activity relatioships of heterocyclic steroidal analogues. Therefore, a series of steroidal[17,16-d]pyrimidines derivatives 3a-p were synthesized according to the method shown in Fig. 3, and their cytotoxic effects on tumor cell lines (HepG2, Huh-7, SGC-7901) were also investigated by MTT colorimetric method, and the possible structure-activity relationships have also been summarized and discussed. These findings can provide interesting information for discovery of potential chemotherapeutic agents.
Results and Discussion
Chemistry
In this study, a series of heterocyclic steroidal[17,16-d]pyrimidines were synthesized via a sequence convenient transformation. The synthetic route for the preparation of these steroidal[17,16-d]pyrimidines derivatives 3a-p is outlined in Fig. 3.
As shown in Fig. 3, the easily available natural steroid DHEA 1 was used as raw materials, which could be readily transferred to various substituted benzylidene-dehydroepiandrosterone derivatives 2a-p by a simple condensation reaction with various aldehydes in the presence of base. Then the treatment of intermediate 2a-p with guanidine nitrate formed the steroidal[17,16-d]pyrimidines 3a-p under the condition of base catalysis. Especially, the products for all these two steps are easy to separate and column chromatography isn’t required. All the newly prepared steroidal[17,16-d]pyrimidines derivatives 3a-p and the intermediates 2a-p gave satisfactory analyses mainly including 1H NMR, 13C NMR and ESI-MS spectrum, and their chemical structures and basic physiochemical properties were summarized in the Supporting Information.
Spectroscopy studies
All structures of target molecules 3a-p were determined by their 1H NMR, 13C NMR and mass spectroscopy, and all these spectral data were in good agreement with their structures. For 1H NMR spectrum, all assignments of the signals are based on their chemical shifts and intensity patterns. As shown in the representative 1H NMR spectrum (Fig. 4), all 1H NMR spectra of compounds 3a-p revealed the distinctive signals of methine proton attached to hydroxyl group, which showed a multiplet or broad singlet at 3.20–3.57 ppm. The signal for protons of alkene bond in DHEA scaffold was resonated as a singlet or doublet between δ 5.19–5.38 ppm. The other set of signals that emerged in their 1H NMR spectrum in the range of 3.19–0.85 ppm were assigned to the protons of DHEA skeleton, and the signals at lower fields in the corresponding 1H NMR spectrum were attributed to the aromatic protons as indicated in the general structures in Fig. 3. The 13C NMR spectra of compounds 3a-p display obvious peaks in the alkyl region indicating the presence of the DHEA scaffold. Other peaks appearing at lower fields were assigned to the aromatic and heterocyclic moiety. The electron spray impact mass spectra (ESI-MS) for compounds 3a-p was measured on a Waters ACQUITY UPLC® H-CLASS PDA (Waters®) instrument, and the ion peak or adduct ions of the compounds were explored. According to the experimental results, the ESI-MS of compounds 3a-p exhibit the obvious molecular peak [M + H]+ with high abundance (100%) in the positive ion mode. In addition, all feature peaks present in the 1H NMR and 13C NMR spectra for target derivatives are described in Supporting Information.
Inhibitory effects on the proliferation of cancer cells for the synthesized compounds
All obtained steroidal[17,16-d]pyrimidines derivatives 3a-p and the steroidal intermediates 2a-p were screened for their potential in vitro cytotoxic activity against HepG2 (human hepatocellular liver carcinoma), Huh-7 (human heptoma), and SGC-7901 (human gastric cancer) cell lines by the standard MTT assay35 using 5-fluorouracil as a control. The preliminary screened results were depicted in Fig. 5 and Table 1, and the IC50 value represents the drug concentration required to inhibit cell growth by 50%.
Generally, as indicated in Fig. 5, most of the heterocyclic steroidal[17,16-d]pyrimidines derivatives 3a-p displayed good cytotoxic activities than the corresponding steroidal intermediates 2a-p. Notably, the compounds 3a, 3b, 3d, 3e, 3f, 3g, 3h, and 3l exhibited excellent inhibitory activities against all three cell lines with 70–82% growth inhibition at the concentration of 40 μg/mL compared to the positive control 5-fluorouracil (58.6–70.5%). From Fig. 5, we also can observe that the typical compounds 3a, 3b, 3d, 3e, 3f, 3g, 3h, and 3l also presented better inhibitory activities than the natural compound DHEA (57.9–69.4%), which indicated these heterocyclic steroidal[17,16-d]pyrimidines can be used as a potential lead compounds for designing of novel anticancer drugs.
Meanwhile, the bioassay revealed many of these heterocyclic compounds mainly including 3a, 3b, 3d, 3e, 3f, 3g, 3h, and 3l had good cytotoxic activities compared to 5-fluorouracil (Fig. 5), so in order to explore the highly potential activities, the IC50 values for these compounds 3a-p were further investigated and compared to 2a-p, DHEA and 5-fluorouracil. The popential inhibitory activities expressed as IC50 values for all compounds are shown in Table 1, which also demonstrated that some of the designed steroidal[17,16-d]pyrimidines derivatives 3a-p exhibited obviously inhibitory activities than the control 5-fluorouracil. Among all the compounds indicated in Table 1, compounds 3a, 3b, 3d, 3e, 3f, 3g, 3h, 3i, 3j, 3k, and 3l showed promising cytotoxic activities (Entries 17, 18, and 20–28) against all three cell lines. Especially, we also can find that compounds 3b, 3g, 3h, 3i and 3l exhibited a certain selective inhibition (Entries 18, 23, 24, 25 and 28) against all three cancer cell lines than the reference 5-fluorouracil. In addition, compound 3b showed the highest inhibitory effect on HepG2, Huh-7 and SGC-7901 cell lines, with an IC50 values of 5.41 ± 1.34, 5.65 ± 1.02 and 10.64 ± 1.49 μM, respectively.
Moreover, the dose-response relationship of cell growth inhibition for highly potential compounds 3b, 3d, 3g, 3l and 5-fluorouracil have been presented in Fig. 6, which indicated that these heterocyclic compounds obviously inhibited HepG2, Huh-7, and SGC-7901 cell proliferation in a concentration-dependent manner. Especially, it should be pointed out that compound 3b containing an ortho-chlorophenyl unit (Entry 18 in Table 1) exhibited the most promising growth inhibitory effects on all three cell lines with the IC50 values of 5.41 ± 1.34, 5.65 ± 1.02 and 10.64 ± 1.49 μM, respectively, which was significantly better than the control 5-fluorouracil and DHEA.
Structure and activity relationships (SARs)
The structure evolution here was to modify DHEA scaffold with pyrimidine ring system (3a-p) and aromatic enones (2a-p), respectively (Fig. 7). According to the in vitro bioassay results shown in Fig. 5 and Table 1, the possible structure-activity profile for these prepared steroidal derivatives can be obtained.
As indicated in Fig. 7, the compounds bearing pyrimidine ring system are general present better inhibition activities than the compounds modified by aromatic enones, which proved the importantance of heterocyclic pyrimidines. In addition, for the compounds containing pyrimidine ring system, the compounds bearing 2-ClPh and 3,4,5-(MeO)3Ph group present the highest potential activities, however, when the substituents R are 2-Py, 3-Py, 4-Py, and 3-PhOPh, which indicate low efficacy. Also, within the series of pyridine derivatives, the compound 3m bearing pyridin-2-yl substituent obviously decrease the cytotoxic activity. On the other hand, for the compounds containing aromatic enone moiety, the results testified that compounds containing 3,4,5-(MeO)3Ph and 2-CF3Ph group exhibited higher activity than the compounds bearing other substituents. From Table 1, we also can find that, within the series of halgen derivatives, it is clear that the ortho-substituted compound is better than the para-substituted compound. In particular, the two compounds containing 3,4,5-(MeO)3Ph group (2l and 3l) all present good inhibitory activities in these two systems, which may be due to the steric size of trisubstituted phenyl group is favourable for the binding to receptor. Taking into account these findings, it can be speculated it would have been more interesting to test the ortho-hydroxy and 3,4,5-trihydroxy substituted derivative, and the special properties of hydroxyl group will be helpful to increase the activity. These preliminary structure-activity relationships were to identify the target steroidal[17,16-d]pyrimidines derivatives that could serve as potential lead antitumor molecules for drug discovery, and the further structural optimization based on these obtained SAR are well under way in our laboratory.
Conclusion
Sixteen steroidal[17,16-d]pyrimidines derived from dehydroepiandrosterone were designed and synthesized via a sequence transformation, and their in vitro inhibitory activities against cell proliferation were evaluated. From the present data it was found that some of these heterocyclic steroidal[17,16-d]pyrimidines displayed significantly good cytotoxic activities against HepG2, Huh-7 and SGC-7901 cell lines compared to the reference 5-fluorouracil, which might be used as promising lead compounds for discovery of novel antitumor molecules. Further structural optimization and possible mechanism on these steroidal[17,16-d]pyrimidines will be investigated in due courses.
Experimental
Synthesis of target compounds
The instrumentation, chemicals, synthetic procedures and characterization were provided in supplementary data.
Biological evaluation
The in vitro cytotoxic activities of these steroidal molecules 2a-p and 3a-p against several human cancer cell lines (HepG2, Huh-7, SGC-7901) were determined using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide] method35, and the general procedures were previously reported in literatures36,37. All the experimental data were analyzed by SPSS software, and the 50% inhibitory concentrations (IC50) of each molecule for the different cell lines were also measured. All biological evaluation was performed in triplicate on three independent experiments, and measurement data were expressed as the mean ± S. D.
Additional Information
How to cite this article: Ke, S. et al. Steroidal[17,16-d]pyrimidines derived from dehydroepiandrosterone: A convenient synthesis, antiproliferation activity, structure-activity relationships, and role of heterocyclic moiety. Sci. Rep. 7, 44439; doi: 10.1038/srep44439 (2017).
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References
Siegel, R., Ma, J., Zou, Z. & Jemal, A. Cancer statistics, 2014. CA Cancer J. Clin. 64, 9–29 (2014).
Nasr, T., Bondock, S. & Youns, M. Anticancer activity of new coumarin substituted hydrazide-hydrazone derivatives. Eur. J. Med. Chem. 76, 539–548 (2014).
Avin, B. R. V. et al. Synthesis and tumor inhibitory activity of novel coumarin analogs targeting angiogenesis and apoptosis. Eur. J. Med. Chem. 75, 211–221 (2014).
Lagoja, I. M. Pyrimidine as constituent of natural biologically active compounds. Chem. Biodiv. 2, 1–50 (2005).
Carter, D. S. et al. Identification and SAR of novel diaminopyrimidines. Part 1: The discovery of RO-4, a dual P2X3/P2X2/3 antagonist for the treatment of pain. Bioorg. Med. Chem. Lett. 19, 1628–1631 (2009).
Piotrowski, D. W. et al. Identification of tetrahydropyrido[4,3-d]pyrimidine amides as a new class of orally bioavailable TGR5 agonists. ACS Med. Chem. Lett. 4, 63–68 (2013).
Chen, P. et al. Synthesis, in vitro antimicrobial and cytotoxic activities of novel pyrimidine-benzimidazol combinations. Bioorg. Med. Chem. Lett. 24, 2741–2743 (2014).
Zhang, Q. et al. Identification of type II inhibitors targeting BRAF using privileged pharmacophores. Chem. Biol. Drug Des. 83, 27–36 (2014).
Gad, H. et al. MTH1 inhibition eradicates cancer by preventing sanitation of the dNTP pool. Nature 508, 215–221 (2014).
Yu, X., Shi, L. & Ke, S. Acylhydrazone derivatives as potential anticancer agents: Synthesis, bio-evaluation and mechanism of action. Bioorg. Med. Chem. Lett. 25, 5772–5776 (2015).
Su, T. et al. Discovenry of novel PDE9 inhibitors capable of inhibiting Aβ aggregation as potential candidates for the treatment of Alzheimer’s disease. Sci. Rep. 6, 21826 (2016).
Venugopala, K. N. et al. Design, synthesis, and computational studies on dihydropyrimidine scaffolds as potential lipoxygenase inhibitors and cancer chemopreventive agents. Drug Des. Dev. Ther. 9, 911–921 (2015).
Nagarajan, S. et al. An eco-friendly and water mediated product selective synthesis of 2-aminopyrimidines and their in vitro anti-bacterial evaluation. Bioorg. Med. Chem. Lett. 24, 4999–5007 (2014).
Ma, W. et al. One-pot synthesis and antiproliferative activity of novel 2,4-diaminopyrimidine derivatives bearing piperidine and piperazine moieties. Eur. J. Med. Chem. 84, 127–134 (2014).
Buron, F., Mérour, J. Y., Akssira, M., Guillaumet, G. & Routier, S. Recent advances in the chemistry and biology of pyridopyrimidines. Eur. J. Med. Chem. 95, 76–95 (2015).
Xu, Y., Yang, J., Ren, L., Hao, S. & Liu, C. Research progress on pyrimidine compounds with insecticidal activities. Agrochemicals 50, 474–478 (2011).
Wu, Q., Song, B., Jin, L. & Hu, D. Research advances in synthesis and antifungal activity of pyrimidine compounds. Chin. J. Org. Chem. 29, 365–379 (2009).
Azab, M. E., Roussef, M. M. & El-Bordany, E. A. Synthesis and antibacterial evaluation of novel heterocyclic compounds containing a sulfonamido moiety. Molecules 18, 832–844 (2013).
Zheng, Z., Chen, J., Liu, G., Li, Y. & Li, Z. Syntheis and herbicidal activity of novel 4-substituted pyrimidinyl-phenyl-sulfonylureas. Chin. J. Pestic. Sci. 14, 607–611 (2012).
Liu, X., Zhang, L., Tan, J. & Xu, H. Design and synthesis of N-alkyl-N’-substituted 2,4-dioxo-3,4-dihydropyrimidin-1-diacylhydrazine derivatives as ecdysone receptor agonist. Bioorg. Med. Chem. 21, 4687–4697 (2013).
Ma, H., Zhang, J., Xia, X., Kang, J. & Li, J. Design, synthesis and herbicidal evaluation of novel 4-(1H-pyrazol-1-yl)pyrimidine derivatives. Pest Manag. Sci. 71, 1189–1196 (2015).
Wang, M., Sun, L., Wan, F. & Jiang, L. Synthesis and phytotoxic activity of novel acylthiourea and 2H-1,2,4-thiadiazolo[2,3-α]pyrimidine derivatives. J. Pestic. Sci. 37, 15–19 (2012).
Li, Y. Syntheris and herbicidal activity of N-[2-(4,6-dimethoxypyrimidin-2-yloxy)benzylidene]substituted amine derivatives. Chin. J. Pestic. Sci. 13, 645–648 (2011).
Zhao, L. et al. Design, synthesis and antifungal activity against Valsa Mali of the triamino substitued triazines bearing aminopyrimidine group. Chem. J. Chin. Univ. 32, 2795–2799 (2011).
Liu, B. et al. Novel pyrimidine-based amphiphilic molecules: synthesis, spectroscopic properties and applications in two-photon fluorescence microscopic imaging. J. Mater. Chem. 17, 2921–2929 (2007).
Zonouzi, A., Hosseinzadeh, F., Karimi, N., Mirzazadeh, R. & Ng, S. W. Novel approaches for the synthesis of a library of fluorescent chromenopyrimidine derivatives. ACS Comb. Sci. 15, 240–246 (2013).
Xue, W., Li, L., Li, Q. & Wu, A. Novel furo[2,3-d] pyrimidine derivative as fluorescent chemosensor for HSO4 − . Talanta 88, 734–738 (2012).
Kubota, Y., Ozaki, Y., Funabiki, K. & Matsui, M. Synthesis and fluorescence properties of pyrimidine mono- and bisboron complexes. J. Org. Chem. 78, 7058–7067 (2013).
Mati, S. S., Chall, S., Konar, S., Rakshit, S. & Bhattacharya, S. C. Pyrimidine-based fluorescent zinc sensor: Photo-physical characteristics, quantum chemical interpretation and application in real samples. Sensor Actuat. B: Chem. 201, 204–212 (2014).
Lednicer, D. Steroid Chemistry at a Glance, John Wiley & Sons Ltd.(2011).
El Kihel, L. Oxidative metabolism of dehydroepiandrosterone (DHEA) and biologically active oxygenated metabolites of DHEA and epiandrosterone (EpiA) – Recent reports. Steroids 77, 10–26 (2012).
Stárka, L., Dušková, M. & Hill, M. Dehydroepiandrosterone: A neuroactive steroid. J. Steroid Biochem. Mol. Biol. 145, 254–260 (2015).
Ke, S., Wei, Y., Shi, L., Yang, Q. & Yang, Z. Synthesis of novel steroid derivatives derived from dehydroepiandrosterone as potential anticancer agents. Anti-Cancer Agents Med. Chem. 13, 1291–1298 (2013).
Ke, S., Shi, L. & Yang, Z. Discovery of novel isatin–dehydroepiandrosterone conjugates as potential anticancer agents. Bioorg. Med. Chem. Lett. 25, 4628–4631 (2015).
Alley, M. C. et al. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res. 48, 589–601 (1988).
Ke, S. et al. Heterocycle-functional gramine analogues: Solvent- and catalyst-free synthesis and their inhibition activities against cell proliferation. Eur. J. Med. Chem. 54, 248–254 (2012).
Shi, L. et al. Anthranilic acid-based diamides derivatives incorporating aryl-isoxazoline pharmacophore as potential anticancer agents: Design, synthesis and biological evaluation. Eur. J. Med. Chem. 54, 549–556 (2012).
Acknowledgements
This work was supported by the Applied Basic Research Program of Wuhan City (2016020101010093) and Hubei Agricultural Science Innovation Centre (2016-620-000-001-039), and the authors also acknowledge the financial support from the National Natural Science Foundation of China (31400153).
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S.K. initiated the idea and performed the chemical synthesis and characterization; L.S. and Z.Z. performed the biological assays; S.K., L.S. and Z.Y. analyzed the results, and S.K. wrote the manuscript.
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Ke, S., Shi, L., Zhang, Z. et al. Steroidal[17,16-d]pyrimidines derived from dehydroepiandrosterone: A convenient synthesis, antiproliferation activity, structure-activity relationships, and role of heterocyclic moiety. Sci Rep 7, 44439 (2017). https://doi.org/10.1038/srep44439
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