Correction
In the recent Research Highlight [1], it has been highlighted that part b of Fig. 1 was incorrectly labelled as “sgRNA + tracrRNA” instead of “sgRNA (crRNA + tracrRNA)”. An updated Fig. 1, including also the amended figure legend has therefore been provided below.
Additionally, in the section entitled, “Rapid and efficient generation of conditional alleles using Easi-CRISPR”, there is a sentence currently written:
“This technique, called efficient addition with ssDNA inserts-CRISPR (Easi-CRISPR), involves targeting by two sgRNAs which flank the endogenous exon and are complexed with Cas9 to form a ribonucleoprotein complex for cellular delivery”.
This is incorrect and should read:
This technique, called efficient addition with ssDNA inserts-CRISPR (Easi-CRISPR), involves targeting by two synthetic sgRNAs (crRNA/tracRNA) which flank the endogenous exon and are complexed with Cas9 to form a ribonucleoprotein complex for cellular delivery.
I apologise for these errors which have been acknowledged and corrected in this Correction article.
Reference
Burgio G. Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology. Genome Biol. 2018;19(1):27.
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The original article can be found online at https://doi.org/10.1186/s13059-018-1409-1.
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Burgio, G. Correction to: Redefining mouse transgenesis with CRISPR/Cas9 genome editing technology. Genome Biol 19, 41 (2018). https://doi.org/10.1186/s13059-018-1424-2
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DOI: https://doi.org/10.1186/s13059-018-1424-2