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Malaria

Methods and Protocols

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  • © 2013
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Overview

Editors:
  1. Robert Ménard

    1. , Malaria Biology and Genetics Unit, Institut Pasteur, Paris, France

  • Presents definitive protocols for malaria researchers
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 923)

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About this book

Over the past ten years, many powerful new techniques have been developed that have dramatically changed malaria research. The second edition of  Malaria: Methods and Protocols expands upon the previous edition with current, detailed techniques for laboratory research. With new chapters on parasite culture techniques, genome manipulation methods, 'omic' approaches, and  techniques for studying the biology of the red blood cell and pre-erythrocytic stages of Plasmodium. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

 

Authoritative and practical,  Malaria: Methods and Protocols, Second Edition, offers a comprehensive set of standard techniques for laboratory  researchers.

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Table of contents (41 protocols)

  2. Red Blood Cell Stages

    1. Front Matter

      Pages 267-267
      Download chapter PDF

    2. Static and Dynamic Imaging of Erythrocyte Invasion and Early Intra-erythrocytic Development in Plasmodium falciparum

      • David T. Riglar, Jake Baum
      Pages 269-280

    3. Flow Cytometry-Based Methods for Measurement of Cytosolic Calcium and Surface Protein Expression in Plasmodium falciparum Merozoites

      • Shailja Singh, Chetan E. Chitnis
      Pages 281-290

    4. Microsphiltration: A Microsphere Matrix to Explore Erythrocyte Deformability

      • Catherine Lavazec, Guillaume Deplaine, Innocent Safeukui, Sylvie Perrot, Geneviève Milon, Odile Mercereau-Puijalon et al.
      Pages 291-297

    5. Atomic Force Microscopy of Plasmodium-Infected Red Blood Cells: Detecting and Localizing Single Molecular Recognition Events

      • Ang Li, Laurent Rénia, Chwee Teck Lim, Bruce Russell
      Pages 299-305

    6. Expressing Full-Length Functional PfEMP1 Proteins in the HEK293 Expression System

      • Anand Srivastava, Yves Durocher, Benoît Gamain
      Pages 307-319

    7. Genome-wide Chromatin Immunoprecipitation-Sequencing in Plasmodium

      • Jose-Juan Lopez-Rubio, T. Nicolai Siegel, Artur Scherf
      Pages 321-333

    8. In Situ Fluorescence Visualization of Transcription Sites and Genomic Loci in Blood Stages of Plasmodium falciparum

      • Liliana Mancio-Silva, Artur Scherf
      Pages 335-351

    9. Bioluminescence Imaging of P. berghei Schizont Sequestration in Rodents

      • Joanna Braks, Elena Aime, Roberta Spaccapelo, Onny Klop, Chris J. Janse, Blandine Franke-Fayard
      Pages 353-368

  3. Pre-Erythrocytic Stages

    1. Front Matter

      Pages 369-369
      Download chapter PDF

    2. Scoring Sporozoite Motility

      • Allison F. Carey, Robert Ménard, Daniel Y. Bargieri
      Pages 371-383

    3. Quantification of Sporozoite Invasion, Migration, and Development by Microscopy and Flow Cytometry

      • Photini Sinnis, Patricia De La Vega, Alida Coppi, Urszula Krzych, Maria M. Mota
      Pages 385-400

    4. Imaging Sporozoite Cell Traversal in the Liver of Mice

      • Joana Tavares, Pauline Formaglio, Alexander Medvinsky, Robert Ménard, Rogerio Amino
      Pages 401-410

    5. Analysis of Liver Stage Development in and Merozoite Release from Hepatocytes

      • Rebecca R. Stanway, Jacqueline Schmuckli-Maurer, Volker T. Heussler
      Pages 411-427

    6. Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence Imaging

      • Takeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard, Shahid M. Khan
      Pages 429-443

  4. Immunity to Plasmodium

    1. Front Matter

      Pages 445-445
      Download chapter PDF

    2. Activation of Human NK Cells by Plasmodium-Infected Red Blood Cells

      • Amir Horowitz, Eleanor M. Riley
      Pages 447-464

    3. Chimeric Parasites as Tools to Study Plasmodium Immunology and Assess Malaria Vaccines

      • Ian Cockburn
      Pages 465-479

    4. Development and Use of TCR Transgenic Mice for Malaria Immunology Research

      • Yun-Chi Chen, Fidel Zavala
      Pages 481-491
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Editors and affiliations

  • , Malaria Biology and Genetics Unit, Institut Pasteur, Paris, France

    Robert Ménard

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Bibliographic information

  • Book title: Malaria

  • Book subtitle: Methods and Protocols

  • Editors: Robert Ménard

  • Series title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-62703-026-7

  • Publisher: Humana Totowa, NJ

  • eBook packages: Springer Protocols

  • Copyright information: Springer Science+Business Media, LLC 2013

  • Hardcover ISBN: 978-1-62703-025-0Published: 19 September 2012

  • Softcover ISBN: 978-1-4939-5958-7Published: 23 August 2016

  • eBook ISBN: 978-1-62703-026-7Published: 19 September 2012

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition number: 2

  • Number of pages: XV, 626

  • Topics: Infectious Diseases, Parasitology

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