Overview
- This book is open access
- Introduces concepts of mutation breeding as an efficient tool to increase the genetic diversity of Arabica coffee
- Presents practical methods on mutation induction and screening for resistance to CLR
- Describes mutation induction and screening for resistance to CLR
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About this book
This open-access book provides a comprehensive overview of current methodologies for improving resistance to leaf rust in coffee, one of the world's most important cash crops and beverages. Coffea arabica L. (Arabica) accounts for about 60% of the world's coffee production. Coffee leaf rust (CLR), caused by the fungus Hemileia vastatrix is the major disease affecting Arabica coffee resulting in losses of over $1 billion annually. The geographical distribution of CLR is expanding due to climate change. Moreover, the genetic improvement of Arabica coffee is constrained due to its very narrow genetic base. This protocol book introduces essential concepts of mutation breeding as an efficient tool to increase the genetic diversity of Arabica coffee and presents practical methods on mutation induction and screening for resistance to CLR. Current breeding approaches, challenges and opportunities for Arabica coffee improvement are briefly reviewed and a survey of common coffee diseases with emphasis on CLR is presented. Practical protocols for mutation induction and screening for resistance to CLR are described, including novel methods for single-cell mutagenesis using in vitro cell and tissue culture techniques and for genome-wide screening of induced mutations using genomics tools. Each protocol chapter has an introduction and is supported by example results. Given the impact of recent CLR epidemics on Arabica coffee production in Latin America, the book is intended to serve as a timely reference and guide for students and researchers in the agricultural sciences, plant pathologists and breeders, as well as growers and end-users interested in producing novel coffee genotypes for genetic studies, breeding, and commercial applications.
Keywords
Table of contents (21 chapters)
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Introduction
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Induced Mutagenesis of in Vitro Tissues and Cells of Coffea arabica L.
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Induced Mutagenesis of Seed and Vegetative Propagules of Coffea arabica L.
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Mutant Phenotyping and CLR Resistance Screening Methods
Editors and Affiliations
About the editors
Maria do Céu Lavado da Silva is a Senior Researcher at the Coffee Rusts Research Center (CIFC), a Unit of Instituto Superior de Agronomia (ISA), School of Agriculture, University of Lisbon. She completed her Ph.D. degree in Agronomic Engineering (1996) at ISA. Her career began in 1984 at CIFC, developing activities on the cytology of plant-microbe interactions. She was awarded Calouste Gulbenkian Foundation Scholarships to the University of Toronto (1994) and Research Institute for Development in Montpellier (1995) and a Syngenta Award for Innovation in Agriculture in Portugal (2008). She hasled the CIFC research team since 2004. She has coordinated and participated in several projects with Institutions from Europe and Coffee Growing Countries. She has published over 90 original research papers. She is regularly involved in international training and supervision of graduate students. She is Vice-President of the Portuguese Society of Plant Pathology, an editorial board member of the journal Agronomy, and a board member of ASIC- Association for Science and Information on Coffee. Her main research focus is on the characterization of mechanisms underlying coffee resistance and susceptibility to the fungi that cause leaf rust and coffee berry disease.
Joanna Jankowicz-Cieslak is a plant molecular biologist and genome scientist with over 18 years’ experience in the field of molecular biology, genomics and crop improvement with the use of mutation breeding techniques. She holds a PhD in biological sciences from the Jagiellonian University in Krakow, Poland. In 2019 she obtained a Habilitation degree in biology from the University of Silesia, Katowice, Poland for the work entitled “Techniques of induction and detection of genetic variability in functional genomics and breeding of plants of special importance in developing countries”. Her research interests focus on the development and adaptation of new tools, protocols and guidelines for mutation induction and discovery in various crops such as coffee. She has extensive experience with induced mutagenesis, in vitro tissue culture, and the application of genomics tools for mutation-assisted breeding. She established the first reverse genetics approach for a vegetatively propagated crop, banana showing that mutations are stably induced and inherited over successive generations. She has (co-) authored over 60 original research papers, monographs and book chapters as well as co-edited four protocol books.
Bibliographic Information
Book Title: Mutation Breeding in Coffee with Special Reference to Leaf Rust
Book Subtitle: Protocols
Editors: Ivan L.W. Ingelbrecht, Maria do Céu Lavado da Silva, Joanna Jankowicz-Cieslak
DOI: https://doi.org/10.1007/978-3-662-67273-0
Publisher: Springer Berlin, Heidelberg
eBook Packages: Biomedical and Life Sciences, Biomedical and Life Sciences (R0)
Copyright Information: IAEA: International Atomic Energy Agency 2023
Hardcover ISBN: 978-3-662-67272-3Published: 02 October 2023
Softcover ISBN: 978-3-662-67275-4Published: 02 October 2023
eBook ISBN: 978-3-662-67273-0Published: 30 September 2023
Edition Number: 1
Number of Pages: XX, 314
Number of Illustrations: 7 b/w illustrations, 92 illustrations in colour
Topics: Plant Pathology, Plant Genetics and Genomics, Agriculture, Plant Sciences