Abstract
Ovarian cancer is amongst the most morbid of gynecological malignancies due to its diagnosis at an advanced stage, a transcoelomic mode of metastasis, and rapid transition to chemotherapeutic resistance. Like all other malignancies, the progression of ovarian cancer may be interpreted as an emergent outcome of the conflict between metastasizing cancer cells and the natural defense mounted by microenvironmental barriers to such migration. Here, we asked whether senescence in coelom-lining mesothelia, brought about by drug exposure, affects their interaction with disseminated ovarian cancer cells. We observed that cancer cells adhered faster on senescent human and murine mesothelial monolayers than on non-senescent controls. Time-lapse epifluorescence microscopy showed that mesothelial cells were cleared by a host of cancer cells that surrounded the former, even under sub-confluent conditions. A multiscale computational model predicted that such colocalized mesothelial clearance under sub-confluence requires greater adhesion between cancer cells and senescent mesothelia. Consistent with the prediction, we observed that senescent mesothelia expressed an extracellular matrix with higher levels of fibronectin, laminins and hyaluronan than non-senescent controls. On senescent matrix, cancer cells adhered more efficiently, spread better, and moved faster and persistently, aiding the spread of cancer. Inhibition assays using RGD cyclopeptides suggested the adhesion was predominantly contributed by fibronectin and laminin. These findings led us to propose that the senescence-associated matrisomal phenotype of peritoneal barriers enhances the colonization of invading ovarian cancer cells contributing to the metastatic burden associated with the disease.
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Introduction
The high morbidity associated with epithelial ovarian cancer (EOC) is due to its ability to rapidly metastasize and colonize the visceral peritoneum. Transformed cells at the primary site are shed into the peritoneal cavity via the movement of peritoneal fluid. Ovarian cancer cells adhere to the lining of the peritonea [1, 2], retract mesothelial cells [3], and actively degrade the underlying and exposed extracellular matrix (ECM) by expressing proteolytic enzymes such as matrix metalloproteinases (MMPs) [2]. Management of EOC involves surgical cytoreduction with chemotherapy provided before or/and after surgery [4]. Cytotoxic therapeutics commonly employed in EOC are carboplatin and doxorubicin, which bind to and alkylate DNA, and paclitaxel, which binds to tubulin and prevents karyokinesis. Treatment with these agents inhibits cell division and ultimately leads to cell death. However, exposure to such drugs may also induce a cytostatic (as opposed to cytotoxic) state in both cancer and primary cells, also known as senescence, wherein the cells are metabolically active but growth-arrested. Therapy-induced senescence (TIS) has been proposed to suppress tumor cell proliferation [5]. On the other hand, it has also been shown to potentiate tumor heterogeneity, resistance [6], and relapse [7]. For instance, TIS in ovarian cancer cells leads to their transient dormancy, which can be reversed to re-initiate proliferation [8, 9]. TIS-associated cytokine secretion (as part of the broader phenomenon of senescence-associated secretory phenotype or SASP) may accentuate infiltration by tumor-associated macrophages (TAMs) in primary tumors leading to greater invasion and metastasis [10,11,12].
Exposure to therapeutic drugs can affect not just the transformed cell niche within a tumor but also the untransformed cellular microenvironment in ways that engender disease progression. Stromal constituents such as endothelial cells and bone marrow-derived cells signal in response to chemotherapeutic exposure to accentuate metastasis [13,14,15,16]. In this context, it is pertinent to study whether senescence in stromal constituents due to drug exposure contributes to cancer cell invasion. Indeed, the accumulation of senescent/aged mesothelia, the cells lining the peritoneal cavity, has been shown to aid ovarian cancer metastasis [17].
In this study, we sought to ask whether chemotherapeutic drugs can drive mesothelial cells to senescence with an unintended potentiation of cancer progression. Using a human-murine hybrid ex vivo system, human mesothelial-cancer coculture system and a Cellular Potts Model (CPM)-based multiscale framework, we found that the senescent mesothelia secrete a unique matrisome that provides a habitable niche for metastasizing cancer cells. A comprehensive understanding of how such a ‘senescence-associated matrisomal phenotype’ leads to higher invasion by ovarian cancer will help design novel therapeutics for the management of metastasis.
Results
Senescent mesothelial monolayers potentiate adhesion of ovarian cancer cells
Parietal peritoneal tissue was dissected from 4 to 6 week Balb/c female mice and cultivated ex vivo as described earlier [18]. Senescence was induced in the explants using 100 nM doxorubicin. Mesothelial cells were harvested from control and senescent murine peritonea and senescence was confirmed by morphological examination (Figure S1A). Control and senescent explants were cultivated with a suspension of GFP-expressing SKOV-3 cells and the adhesion of the latter assessed (Fig. 1A). SKOV-3 cells were observed to attach faster to the senescent murine peritoneum than untreated controls (Fig. 1Bi-ii; statistical significance shown in Fig. 1Biii). To confirm this observation, an immortalized human mesothelial cell line MeT-5A was made senescent using 100 nM doxorubicin. The senescent mesothelial cells showed an enlarged, flattened, and fragmented nuclear morphology (Figure S2A). The senescent MeT-5A monolayers stained positive for SA-β-Gal staining, the most common marker for senescence (Figure S2B). We also observed higher expression of the molecular marker p21 in senescent MeT-5A monolayers (Figure S2C). An adhesion assay similar to that in Fig. 1A was performed on RFP-expressing senescent MeT-5A monolayers (Fig. 1C). We observed significantly higher adhesion of GFP-expressing SKOV-3 cells on senescent MeT-5A monolayers (Fig. 1Di-iii). This effect was further validated by performing the adhesion assay on senescent MeT-5A monolayers using GFP-expressing OVCAR-3 cells (Figure S3A). We observed significantly higher adhesion of OVCAR-3 cells on senescent MeT-5A monolayers than on control MeT-5A counterparts (Figure S3B).
Ovarian cancer cells proliferate faster within a senescent mesothelial microenvironment
We asked if ovarian cancer cells not just adhered to, but managed to grow better in, a mesothelial microenvironment with therapy-induced senescence. To investigate this, we performed time-lapse epifluorescence microscopy on monolayers of RFP-expressing control and senescence-induced MeT-5A mesothelia cultured with suspended GFP-expressing SKOV-3 cells (Figs. 2A-B; see also videos S1 and S2, respectively). We observed that the adhered SKOV-3 cells could not only proliferate better within the senescent MeT-5A monolayers (Fig. 2Ci) but also clear senescent monolayers more efficiently (Fig. 2Di). After 72 h of cancer cell addition, we found a significantly higher area occupied by SKOV-3 cells within the senescent MeT-5A monolayers compared to the area occupied by SKOV-3 cells within control MeT-5A monolayers (Fig. 2Cii). We also observed a statistically significant reduction in the area of senescent MeT-5A monolayers compared to control MeT-5A monolayers when both were cultured with SKOV-3 cells (Fig. 2Dii). We did not observe any notable difference in acellular spaces between control and senescent mesothelial monolayers prior to the adhesion of cancer cells, which suggested that clearance rather than available adherable space could play a role in a greater cancer cell occupancy within senescent cell populations.
To confirm that the clearance of senescent MeT-5A monolayers is indeed caused by SKOV-3 cells, a senescent monolayer without SKOV-3 cells was also imaged for 72 h (Figure S4A; see also video S3). We observed a significant reduction in the area occupied by senescent MeT-5A monolayers in the presence of SKOV-3 cells compared to SKOV-3-less controls (Fig. 2Di and Figure S4B). To confirm the ability of ovarian cancer cells to clear, and proliferate amidst, senescent mesothelia, GFP-expressing OVCAR-3 cells were cultivated with senescent and control MeT-5A monolayers for 60 h (Figure S5A and S5B). We observed higher proliferation of OVCAR-3 cells within senescent MeT-5A monolayers (Figure S5Ci-ii) and a significant reduction in the area occupied by senescent MeT-5A monolayers compared with control MeT-5A monolayers (Figure S5Di-ii). We also observed a substantial decrease in the area occupied by senescent MeT-5A monolayers cultured with OVCAR-3 cancer cells compared with senescent MeT-5A monolayers where with OVCAR-3 cells were not added (Figure S5E-F and Di).
Multiple cancer cells surround and clear senescent mesothelia independent of confluence
Extrusion of cells within populations is well known across developmental and oncological contexts and is often driven through cell-density dependent mechanisms [19, 20]. We asked if the clearance of mesothelia was achieved by cancer cells only when both were part of a jammed confluent population. To answer this question, we examined our epifluorescence time-lapses and observed senescent cell extrusions by SKOV-3 cells even in sub-confluent conditions (Fig. 3A). We found that the total number of extrusions in senescent MeT-5A monolayers with SKOV-3 cells was significantly higher than the total number of extrusions in control MeT-5A monolayers with SKOV-3 cells and senescent MeT-5A monolayers without SKOV-3 cells (Fig. 3B). Moreover, we observed that a significant number of extruded senescent MeT-5A were surrounded and impacted by more than one SKOV-3 cell before their clearance (Fig. 3C). To our surprise, we also observed that senescent cells distant from SKOV-3 cells showed a lower frequency for extrusion (similar to non-senescent cells in controls distant from SKOV-3 cells. This suggested that the extrusion of senescent mesothelial cells was strongly correlated with their proximity with ovarian cancer cells.
To further characterize cellular neighborhoods within coculture monolayers, we determined the total number and type of neighbors for senescent and non-senescent mesothelia within cocultures. Due to their larger surface area, the number of neighbors for senescent cells is naturally higher (~ 4–5 cells) than for non-senescent cells (~ 2–3 cells). Nevertheless, the proportion of neighboring cells being cancer cells was found to be significantly higher for senescent mesothelia than their counterpart non-senescent cells in the same fields (Fig. 3D).
Our observation that cancer cells can clear mesothelia independent of cell crowding effects suggested that confluence dependent cell extrusion may not be operative here. This surmise was reinforced by our observation of a closer localization of cancer cells to senescent mesothelia. To shed light on the intercellular interactions that allow confluence-agnostic senescent cell shedding, we employed a multiscale virtual tissue simulation approach using the Cellular Potts Model (CPM)-based computational framework Compucell3D. A monolayer consisting of senescent, non-senescent, and cancer cells was simulated initially, incorporating differences in their cell proliferation rates and relative cell size. The simulation period in Monte-Carlo steps (MCS) was calibrated with experiments by comparing the growth of cancer cells in the experiments to the growth rate of cancer cells in simulations. The size and number of each cell type was quantified based on their initial starting conditions in experiments. Our first model (Fig. 4Bi; video S4) incorporated no other interactive rule. It was, therefore, not surprising that the first model resulted in a confluent monolayer without any colocalization of cancer cells and senescent cells and without any clearance of the latter. Based on our observations in Fig. 3A, we incorporated an additional rule (Model 2, Fig. 4Bii; video S5), wherein clearance of senescent cells could occur if they share > = 55% of their perimeter with cancer cells. This did result in the extrusion of senescent cells, but only after cancer cells had proliferated enough to create a confluent cocultured monolayer. Therefore, clearance occurred much after confluence had been reached. This led us to hypothesize that an effective attractive force may exist between senescent and cancer cells, driving them to localize close to each other (consistent with our experimental observations of their proximity). Solely implementing such an attractive force (through decreasing the contact energy of adhesion between the senescent and cancer cells; CEnew = 15, while CEold = 30) was insufficient for mesothelial clearance (Model 3, Fig. 4Biii; video S6). However, the simultaneous implementation of adhesive attraction between senescent and cancer cells and the rule for extrusion (Model 4, Fig. 4Biv; video S7) brought about clearance even when the monolayers were sub-confluent, similar to what we observed in experiments (as shown in Fig. 4C, D). We next asked whether senescent cells secreted any chemoattractants that could bring about a closer localization of cancer cells around senescent cells.
A senescent-associated matrisomal phenotype (SAMP) provides a better substratum for the adhesion and spread of ovarian cancer cells
Senescent cells are well known to secrete a gamut of soluble cytokines that may regulate the attraction and proliferation of cells surrounding them, a phenomenon referred to as senescence-associated secretory phenotype [21, 22]. To verify if the secretome of senescent mesothelia could exert such an effect on ovarian cancer cells, we cultivated SKOV-3 cells in the conditioned medium collected from senescent (and control non-senescent) cells for 36 h (Figure S6Ai). We did not observe any significant difference between the proliferation of SKOV-3 cells with conditioned media from senescent and non-senescent mesothelia (Figure S6Aii). We then extended our investigation to the extracellular matrix (ECM) secreted by senescent cells to check the proliferation of the adhered SKOV-3 cells (Figure S6Bi). We did not observe a significant change in the proliferation of SKOV-3 cells in the senescent and non-senescent extracellular matrices (Figure S6Bii). However, to our surprise, SKOV-3 cells occupied a larger proportion of decellularized senescent cell ECM-covered area than non-senescent controls (Fig. 5A). Upon quantification of cell number and size, we found that the greater occupancy was due to the increased number and surface area of adhered SKOV-3 on the decellularized senescent mesothelial ECM compared with control cell ECM, respectively. (Fig. 5Bi-ii). Moreover, migrating SKOV-3 cells showed higher velocity and directionally persistent migration on senescent mesothelial ECM than non-senescent controls (Fig. 5C i-ii; see also videos S8 and S9). Consistent with our observations of SKOV-3, OVCAR-3 cells did not show any difference in proliferation upon cultivation in the presence of conditioned media from senescent and non-senescent mesothelia (Figure S7A). We also observed no difference in their proliferation when grown on decellularized senescent versus non-senescent ECM (Figure S7B); however, we found that a significantly higher number of OVCAR-3 cells adhered to, and spread to a greater extent on, senescent mesothelial ECM (Figure S8A-Bi-ii). In contrast, the adhesion and motility of SKOV-3 cells cultivated on plastic substrata in the conditioned medium of untreated and senescent mesothelia were similar (Figure S9A-C; see also Video S10 and S11). We then asked what proteins within senescent ECM may be responsible for their ability to enhance adhesion and motility of cancer cells.
Senescent mesothelial cells secrete higher levels of fibronectin, laminins, and hyaluronic acid
Cells adhere to the extracellular matrix through interactions mediated by their cell surface receptors. Matrix adhesion is predominantly mediated by interactions between integrins on the cell surface and epithelia-secreted laminin [23] and the stromal glycoprotein fibronectin [24, 25] on the substrata, but also through interactions between cell surface CD44 and hyaluronan [26, 27] (Fig. 6A). Therefore, we first performed immunofluorescence on senescent and control MeT-5A monolayers to characterize the differences in levels of laminins and fibronectin within the two monolayers. We observed significantly higher levels of fibronectin (Fig. 6Bi) and pan laminin (Fig. 6Ci) in senescent MeT-5A monolayers compared to control counterparts. Quantitative RT-PCR confirmed the same and showed a 1.8-fold change in fibronectin gene expression (Fig. 6Bii) and a 4.5-fold change in the expression of the gene encoding laminin B3 (Fig. 6Cii) for senescent mesothelia compared with controls. The strong presence of fibronectin in the senescent mesothelial microenvironment was even observed after the culture was decellularized (Figure S10i-ii), suggesting senescent mesothelia do secrete higher levels of fibronectin and other ECM proteins that constitute the adhesive substratum around themselves. We performed Alcian blue staining on senescent and control MeT-5A monolayers to characterize the differences in the expression of acidic mucins within the two monolayers (Fig. 6Di). We observed significantly higher levels of hyaluronic acid glycosaminoglycans in senescent MeT-5A monolayers than in control counterparts (Fig. 6Dii).
Increased expression of fibronectin and laminins aid in the greater adhesion of ovarian cancer cells on senescent extracellular matrix
Ovarian cancer cells are known to bind hyaluronic acid via the CD44 receptor that aids in the adhesion of cancer cells to the peritonea [28]. Ovarian cancer cells are known to adhere to the peritoneal mesothelia by canonical interactions between β-1 integrins and fibronectin [29]. Therefore, to study which of these may be contributing canonically to cell adhesion, we used a soluble cyclic RGD peptide in adhesion assays on senescent and non-senescent matrices as an allosteric inhibitor to hinder the canonical fibronectin- and laminin-integrin binding (Fig. 7A i–ii). As expected, with the addition of cyclic RGD peptide, we observed a decline in the number of adhered SKOV-3 cells on the senescent ECM (Fig. 7B). In fact, at 20 μg/mL of cyclic RGD peptide, the adhesion of SKOV-3 cells on senescent ECM was even lower than that on the non-senescent ECM (Fig. 7C), suggesting that the greater adhesion of cancer cells on senescent ECM comprises adhesive interactions between their cell-surface integrins and its matrix ligands.
Discussion
Aging and senescence have been proposed to contribute to a higher incidence of epithelial ovarian cancer [30, 31]. In fact, secretory cytokines of aged mesothelia have, through elegant experiments, been shown to account for a two-fold increase in peritoneal metastasis in aged patients [17]. However, cellular senescence is not just a consequence of multiple rounds of replication associated with aging, but also of exposure to DNA-adducing chemicals such as doxorubicin and carboplatin. Therefore, it is imperative to investigate how such therapy-induced senescence (TIS) may exacerbate the progression of ovarian cancer, since it may impact decisions by clinicians on the administrative regimen of such drugs [32, 33].
Our investigations led us to observe that senescent mesothelia enhanced not just the adhesion of suspended ovarian cancer cells but also their proliferation and movement once the cells attached and were part of the monolayer. The proliferation is likely caused by the enhanced clearance of senescent mesothelia as was evidenced through time lapse imaging. Surprisingly though, the senescent mesothelial extrusion was not a consequence of crowding within the population of mesothelial and cancer cells. Imaging revealed that the cancer cells preferentially localized proximal to senescent mesothelia and cleared them even under sub-confluent conditions. Computer simulations have been shown to be useful in predicting the diversities in phenotype of interacting cells with distinct traits [34,35,36,37]. We used a Cellular Potts Model-based simulation framework to explore the interaction between senescent, non-senescent, and transformed cells. Our computational results indicate that to achieve the rate of clearance of senescent cells observed in experiments required both a mechanism of ‘active extrusion’ of senescent cells by cancer cells and high adhesive interactions between senescent mesothelia and cancer cells. None of these mechanisms were sufficient without the other. To our surprise, we found that the senescent mesothelia secreted an ECM that was preferentially adhesive to cancer cells (in comparison to the non-senescent mesothelial ECM). Senescent mesothelial ECM was shown to be composed of higher levels of fibronectin, laminin and hyaluronic acid, all of which are well known adhesive ECM constituents. Cancer cells not just adhered faster on senescent ECM, they also spread better. In addition, both their velocity and persistence of migration were greater on senescent ECM, suggesting a coupling between these two traits (as has been proposed for mesenchymal migration) [38, 39]. We define these cellular behaviors collectively as senescence-associated matrisomal phenotype (or SAMP, in semantic consonance with the well-known senescence associated secretory phenotype (SASP) [40, 41]).
Ovarian cancer cells that were treated with conditioned media from senescent mesothelia did not exhibit a significant difference in adhesion, motility, or proliferation compared with senescent controls, leading us to surmise that the effects of mesothelial senescence on cancer cell phenotype are predominantly matrisomal and not due to secreted soluble factors. However, the time course of these experiments was short, disallowing a rigorous discounting of secretory effects especially on cell proliferation and motility. Experiments on non-senescent mesothelia show their clearance by cancer cells is dependent on forces generated by myosin [42]. Although not explored in this manuscript, the proximal localization of mesenchymal cancer cells near senescent mesothelia before the latter are extruded suggests a similar mechanism is at play here and will be experimentally probed in the future.
Prominent experiments describing universal coupling of speed and persistence (UCSP) as being canonical to motile cells have been performed on fibronectin-coated substrata [39, 43]. This led us to probe and subsequently demonstrate that ovarian cancer cells adhere strongly to senescent ECM likely through adhesive interactions between integrins and RGD-containing ECM glycoproteins such as laminins- and fibronectin. Which tertiary laminin (or any other novel ECM protein) is specifically involved remains to be discovered and will likely be revealed through quantitative mass spectrometric analyses on the senescent and non-senescent matrisomes, followed by validation through perturbations in expression of identified target proteins. In our study, not just ECM proteins, but the non-conjugated glycosaminoglycan hyaluronan is also upregulated in senescent mesothelia. In this manuscript, we have not tested its role in cancer cell adhesion. Using genetic perturbation and pharmacological inhibition, we aim to probe how interactions likely with CD44 may also potentiate adhesion of cancer cells on senescent peritoneal environments, after verifying if such interactions are crucial for the induction of senescence.
In spite of the stated limitations of our experimental study, it is evident that the accumulation of senescent mesothelial cells as an unintended consequence of chemotherapeutic exposure aids ovarian cancer progression. The supplementation of chemotherapy with senolytics or targeted inhibitors of cell-ECM adhesion may help mitigate such pro-metastatic effects [44, 45]. On a broader note, these observations argue for a holistic approach to cancer management, one which targets not just cancer cells but their untransformed cellular allies within the tumor microenvironment.
Materials and methods
Cell culture
MeT-5A cells purchased from ATCC were maintained in Medium 199 media (HiMedia; AL014A) along with 10% fetal bovine serum (Gibco; 10,270), SKOV-3 cells were maintained in McCoy’s 5A media (HiMedia; AL057A) along with 10% fetal bovine serum (Gibco; 10,270), and OVCAR-3 cells were maintained in RPMI-1640 media (HiMedia; AL162A) along with 20% fetal bovine serum (Gibco; 10,270) in a 5% carbon dioxide, 37° C temperature humidified incubator.
Senescence induction
Non-senescent MeT-5A cells were seeded in 60 mm dishes from an early passage of healthy MeT-5A cells growing and allowed to adhere for 18 h. Then they were treated with 100 nM of doxorubicin (Cayman chemicals; 15,007) for 48 h [46, 47]. The control sample was added with a proportionate volume of DMSO to negate the effect of solvent on MeT-5A cells present in the treated samples. The control and treated samples were cultured in drug-free media for 24 h and then checked for senescence [47].
BALB/c female mice (4–6 weeks old) were sacrificed for ex-vivo adhesion experiments. Mice were euthanized by cervical dislocation and confirmed dead by cessation of respiration. The peritoneal membrane was surgically dissected and cut into two parts and cultured in Medium 199 media (HiMedia; AL014A) along with 10% fetal bovine serum (Gibco; 10,270) with and without 100 nM of doxorubicin to generate senescent and non-senescent murine peritonea, respectively. GFP-expressing SKOV-3 were suspended on top of the murine peritonea and were allowed to adhere for 30 min. The murine peritonea were washed with 1 × PBS and imaged by using an Olympus IX83 inverted epifluorescence microscope at 10X and 4X magnification. The images were analyzed and the number of cells adhered to the matrices was counted using the “analyze particles” feature in ImageJ. Murine cells were isolated from senescent and non-senescent peritonea by incubating them in 0.25% trypsin solution for 8 min, followed by streaking over the tissue with a tip to dislodge the cells from the tissue. Extracted cells were allowed to adhere for 48 h and used for morphological examination for senescence.
Cell staining
MeT-5A cells were directly seeded in an eight-well chambered cover glass for staining the DNA and F-actin. Monolayers of senescent and non-senescent MeT-5A cells were fixed using 4% formaldehyde (24,005; Thermo Fisher Scientific) at 4 °C for 20 min. After fixation, the cells were taken for further processing or stored in 1 × PBS at 4 °C. Permeabilization was achieved using 0.5% Triton X-100 (MB031; HiMedia) for 90 min at RT. Alexa Fluor 488 Phalloidin (Invitrogen; A12379) dissolved in 0.1% Triton X-100 was incubated overnight at 4 °C in dark. This was followed by washes using 0.1% Triton X-100 in PBS (5 min × 3). Samples were incubated with DAPI (D1306; Thermo Fisher Scientific) dissolved in 0.1% Triton X-100 for 10 min in dark at RT. Subsequent processing was carried out in the dark. These included washes using 0.1% Triton X-100 in PBS (5 min × 2). The images were captured at 10 × magnification using an Olympus IX83 inverted epifluorescence microscope and an Olympus IX83 inverted fluorescence microscope fitted with Aurox structured illumination spinning disk setup. Images were processed and analyzed using ImageJ.
Cytochemical detection of SA-β-Gal
MeT-5A cells were fixed with 0.2% glutaraldehyde in PBS for 15 min at room temperature (RT). The cells were washed twice with PBS and incubated overnight in fresh SA-β-gal staining solution containing 1 mg/ml X-Gal, 5 mM potassium ferrocyanide, 5 mM potassium ferricyanide, 150 mM NaCl, 2 mM MgCl2, and 0.1 m phosphate buffer, pH 6.0 in darkness at 37° C without CO2 [48]. Upon development of the bluish-green stain post-incubation period, images were visualized using a phase-contrast microscope and captured on a Magcam DC camera using MagVision software. Quantitative analysis was performed on the images using ImageJ.
Mesothelial clearance assay
Non-senescent and senescent RFP-expressing MeT-5A cells were trypsinized and seeded (40,000 in number) in eight well-chambered cover glasses. Sub-confluent monolayers were allowed to form for the next 24 h and GFP-expressing SKOV-3 cells were added to the non-senescent and senescent monolayers. The assay was performed with Medium 199 media (HiMedia, AL014A) along with 10% fetal bovine serum (Gibco, 10,270). Time-lapse imaging was subsequently performed for 72 h right after the addition of SKOV-3 cells at 30 min intervals using a Tokai Hit stage-top incubator with image acquisition through an Orca Flash LT plus camera (Hamamatsu) on an Olympus IX73 microscope. The videos were stitched using ImageJ and the same was used to calculate the change in the area of MeT-5A monolayers and SKOV-3 cells in the same fields (shown in figure S11).
Quantitative real-time PCR
Quantitative real-time PCR was performed for common matrisomal genes, where GAPDH was used as an internal control for the normalization of RT qPCR data. Total RNA was isolated using RNAiso Plus from MeT-5A monolayers, after which 1 μg of total RNA was reverse transcribed to cDNA using Verso cDNA Synthesis kit as per the manufacturer’s protocol (AB-1453; Thermo Fisher Scientific). Real-time qPCR was performed on QIAGEN Rotor-Gene Q System (QIAGEN) using a standard two-step amplification protocol followed by a melting curve analysis. The amplification reaction mixture (total volume of 10 μL) contained 10 ng of cDNA, 5 μL 2 × DyNAmo Flash SYBER Green master mix, and 0.25 μM of the appropriate forward and reverse primer. Primer sequences of GAPDH and matrisomal genes are mentioned in the Table S1. Relative gene expression was calculated using the comparative Ct method, and gene expression was normalized to non-senescent MeT-5A cells. Appropriate no template and no RT controls were included in each experiment. All the samples were analyzed in triplicates and repeated at least three times independently.
Immunostaining and image acquisition
MeT-5A cells were directly seeded in an eight-well chambered cover glass for immunostaining. Non-senescent and senescent MeT-5A cells were fixed using 4% formaldehyde (24,005; Thermo Fisher Scientific) at 4 °C for 20 min. After fixation, the cells were taken for further processing or stored in 1 × PBS at 4 °C. Permeabilization was achieved using 0.5% Triton X-100 (MB031; HiMedia) for 90 min at RT. Blocking was achieved using PBS with 0.1% Triton X-100 and BSA (MB083; HiMedia) for 45 min at RT. Primary antibody incubation was carried out overnight at 4 °C. This was followed by washes using 0.1% TritonX-100 in PBS (5 min × 3). Secondary antibodies and Alexa Fluor 488/568 phalloidin (Invitrogen; A12379; A12380) were incubated with the sample at RT for 2 h under dark conditions. DAPI (D1306; Thermo Fisher Scientific) was added to the samples and washed away after 15 min. Subsequent processing was carried out in the dark. These included washes using 0.1% Triton X-100 in PBS (5 min × 3). Images were captured at 10 × magnification using an Olympus IX83 inverted epifluorescence microscope. Images were processed and analyzed using ImageJ. The antibodies used in our studies are against pan-Laminin (ab11575) and fibronectin (E5H6X). Negative controls in each case were through the omission of the primary antibody.
Alcian blue staining
MeT-5A cells were directly seeded in an eight-well chambered cover glass for alcian blue staining. Non-senescent and senescent MeT-5A cells were fixed using 4% formaldehyde (24,005; Thermo Fisher Scientific) at 4 °C for 20 min. After fixation, the cells were washed three times with 1 × PBS and then taken for further processing or stored at 4 °C. While staining for Hyaluronic acid and acid mucins, the MeT-5A monolayers were incubated with 3% acetic acid solution for three minutes and then with the alcian blue solution (1% w/V alcian blue, 8GX (HiMedia, TC359-10G) in 3% acetic acid solution at 2.5 pH) for 30 min at 37 °C. Perform three 1 × PBS washes for 5 min each. Upon development of the bluish-green stain, images were visualized using a phase-contrast microscope and captured on a Magcam DC camera using MagVision software. Quantitative analysis was performed on the images using ImageJ.
Conditioned media treatment
The seeded non-senescent MeT-5A cells were made senescent by incubation with 100 nM of doxorubicin (Cayman chemicals; 15,007) for 48 h. The media along with the remaining doxorubicin was removed and the senescent monolayer was washed with 1 × PBS. Senescent monolayers were incubated with doxorubicin-depleted media for another 24 h and the conditioned media (CM) was collected. Non-senescent MeT-5A monolayers were also incubated with media for 24 h and the CM was collected. A 1:1 mixture of CM from senescent/non-senescent MeT-5A and Medium 199 media (HiMedia; AL014A) along with 10% fetal bovine serum (Gibco; 10,270) were used for conditioned media growth assay with SKOV-3 cancer cells.
Senescent and non-senescent matrix preparation
Senescent and non-senescent MeT-5A cells were seeded at high confluency on chamber wells with coverslip bottoms. The cells were allowed to adhere and lay down the extracellular matrix for the next 36 h. The confluent MeT-5A monolayers were then washed with 1 × PBS twice and decellularized using a 20 mM solution of NH4OH in Milli-Q water for 7–10 min [49]. The cellular debris was gently removed by giving 2–3 washes with Milli-Q water. The extracted extracellular matrices laid on the chamber wells were used for adhesion assays.
Adhesion assay with RGD peptide
The SKOV-3 cells (5000 in number) suspended in Mcoy’s 5A media (HiMedia; AL057A) along with 10% fetal bovine serum (Gibco; 10,270) were added to the decellularized senescent and non-senescent matrices and allowed to adhere on the matrix for 25 min. For adhesion assay with RGD peptide, the media also had cyclic RGD peptide (Sigma; G1269-1MG) at 10 and 20 μg/mL concentrations. After incubation, the wells were washed with 1 × PBS twice and immediately imaged by using an Olympus IX83 inverted epifluorescence microscope at 10 × magnification. The images were analyzed and the number of cells adhered to the matrices was counted using the “analyze particles” feature in ImageJ. The attached cells were also time-lapsed for 24 h using a Tokai Hit stage-top incubator with image acquisition through an Orca Flash LT plus camera (Hamamatsu) on an Olympus IX73 microscope. The data were analyzed using a manual tracking plugin in FIJI and Ibidi’s chemotaxis and migration tool to see differences in displacement and velocity of SKOV-3 cells on senescent and non-senescent matrices (can be seen in videos S8 and S9).
Compucell 3D model for the clearance of senescent mesothelia
Compucell3D (CC3D) is based on the Cellular Potts Model (CPM), also known as the Glazier-Graner-Hogeweg (GGH) model. It has been utilized extensively for computational models of the collective behavior of cellular structures. The CPM is a lattice-based discrete model in which spatiotemporal development is simulated via an energy minimizing procedure [34, 50]. Each cell consists of a collection of lattice sites (pixels). Each configuration is associated with an effective energy, or Hamiltonian (H), which is calculated based on properties such as volume, surface area, contact energies, or external properties. Time evolution is simulated via Monte Carlo Steps (MCS) that involve random changes of lattice site occupations and the changes that decrease the energy are more likely than those that increase it. The Hamiltonian of the system is formulated as:
where \(i,j\) are lattice sites, \({\sigma }_{i}\) is the cell at site i, \(\tau \left(\sigma \right)\) is the cell type of cell \(\sigma\), J is the coefficient determining the adhesion between two cells of types \(\tau \left(\sigma \right)\), \(\tau \left(\sigma {\prime}\right)\), \(\delta\) is the Kronecker delta, v(\(\sigma\)) is the volume of cell \(\sigma\), V(\(\sigma\)) is the target volume, and \(\lambda\) is a Lagrange multiplier determining the strength of the volume constraint. We utilized a simple two-dimensional model 140*140*1 using a square pixel lattice to find and understand the effect of factors sufficient to bring a considerable decline in the senescent mesothelia area as observed in the mesothelial clearance experiments that were recorded using time-lapse epifluorescence videography. The simulation time was set to 8000 MCS as per the corresponding normalized length of the experimental time-lapse videos. The lattice is composed of the medium, senescent cells (SEN), non-senescent cells (NON_SEN), ovarian cancer cells (CANCER), and a frozen wall that mimics a closed system such as enclosed peritoneal cavity (shown in figure S12). This simple model has four variations with the contact energies between cancer and senescent cells to be either 15 (greater adhesion) or 30 (same as other contact energy combinations) and activation or deactivation of the active extrusion rule (shown in figure S13). The other important parameters used in the model and their input values are available at Table S2. To compare the simulation time to the timescale of senescent mesothelial clearance by ovarian cancer cells, a calibration was performed. The division time of ovarian cancer cells in experiments (SKOV-3 cells) was 18 h and the division time of ovarian cancer cells in simulations was 750 MCS. This factor was used to calibrate the simulations time to real-time and the comparison between the computational model and senescent mesothelial clearance experiments was made.
Statistical analysis
The experiments were repeated at least three times and unpaired parametric t-test with Welch’s correction were used for statistical testing unless otherwise stated in the figure legends. The graphs were plotted using GraphPad Prism 8.0.2 and p-values < 0.05 was considered statistically significant.
Data availability
The raw data for all experiments and simulations will be made available upon request.
Code availability
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Acknowledgements
The authors would like to thank Abraham Binoy Mathew for technical help with senescence induction.
Funding
This work was supported by the Wellcome Trust/DBT India Alliance Fellowship/Grant [IA/I/17/2/503312] awarded to RB. It was also supported by the John Templeton Foundation (#62220) (to RB and TG) and by the Department of Biotechnology, India [BT/909 PR26526/GET/119/92/2017] (to RB). DKS acknowledges support from Science and Engineering Research Board (SERB), India (#CRG/2020/000239) and financial support from IISc. BVT acknowledges KVPY for the student scholarship [SX(E-SC)-1711005]. The opinions expressed in this paper are those of the authors and not those of the John Templeton Foundation. MB is supported by the DBT JRF fellowship from the Department of Biotechnology, India.
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RB, DKS and BVT designed the experiments. BVT and MB carried out the experiments and simulations. RB, DKS, MB, TG and BVT analysed the results of the experiments and simulations. RB and BVT wrote the manuscript. RB, DKS, TG, MB and BVT edited the manuscript.
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Thapa, B.V., Banerjee, M., Glimm, T. et al. The senescent mesothelial matrix accentuates colonization by ovarian cancer cells. Cell. Mol. Life Sci. 81, 2 (2024). https://doi.org/10.1007/s00018-023-05017-x
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DOI: https://doi.org/10.1007/s00018-023-05017-x