Abstract
Drug-coated balloons have proved to be an effective technology in percutaneous transluminal angioplasty in treating peripheral artery disease. Paclitaxel-based coating is mainly used. Solutions to such problems as drug loss and inefficient drug release during operations, however, have not been found yet. This study aims to explore the activity of a newly designed paclitaxel-coated balloon in vitro using pectin as the excipient (pectin-paclitaxel) compared with the commercially available shellac excipient balloon, and to characterize the novel nanoparticle paclitaxel-coated balloon with peptide (Arg-Gly-Asp-Ser, RGDS) derivative RGDS-OC8H17 (pectin-RGDS-OC8H17-paclitaxel). Two coating solutions, pectin-paclitaxel and pectin-RGDS-OC8H17-paclitaxel, were successively designed and prepared. The morphology of both coating solutions was first characterized compared with the control group, the commercially available paclitaxel-coated balloon. Then the in vitro experiments were conducted to determine the drug-releasing profiles of both pectin-paclitaxel and pectin-RGDS-OC8H17-paclitaxel coatings. The pectin-RGDS-OC8H17-paclitaxel-coated balloon was smoother and more homogeneous compared with the commercially available paclitaxel-coated balloon and the pectin-paclitaxel-coated balloon. This difference was more obvious when paclitaxel was at low concentration. During the in vitro trial, the drug-releasing curve of the pectin-RGDS-OC8H17-paclitaxel model showed an adjustable paclitaxel-releasing: more than 90% of the paclitaxel released in 2 h at 300 rpm and more than 99% released in 10 min at 1200 rpm. Compared to the performance of the current commercially available shellac excipient products and the pectin-paclitaxel coating, pectin-RGDS-OC8H17-paclitaxel coating provided higher drug-releasing speed. However, the clinical outcomes of this finding need to be further demonstrated. Paclitaxel-coated balloons as an effective therapeutic strategy currently in treating peripheral arterial disease need to be further improved in terms of its efficiency in anti-proliferative drug delivery and release. The pectin-RGDS-OC8H17-paclitaxel coating solution developed in this study exhibited excellent drug-releasing properties. Further experiments are still needed to demonstrate the performance of this novel drug-coated balloon in vivo and its clinical importance.
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Introduction
Epidemiological data have shown that more than 2 million people surfer from peripheral artery disease (PAD) worldwide (Criqui Michael 2015). As for symptomatic lower extremity peripheral artery disease in particular, percutaneous transluminal angioplasty (PTA) has become the optimal therapy in most cases (Criqui et al. 1989; Gerhard-Herman Marie et al. 2017).
With the increasing use of PTA, drug-coated balloons (DCBs) or specifically paclitaxel-coated balloons (PCB) are proved in recent years a more effective technology in PAD treatment and restenosis prevention via dilating narrow arteries and delivering anti-proliferative drugs to target lesions (Sarode et al. 2014; Gongora et al. 2015; Scheinert et al. 2014). Meanwhile, such problems as during-operation drug loss and inefficient drug release during balloon inflation need to be further solved (Heilmann et al. 2010; Sur et al. 2011).
It is known that PCB mainly functions with paclitaxel, an active anti-proliferative drug, and its carrier or matrix, the excipient which serve as a medium to promote drug transfer into the target lesion sites. Common excipients include triethyl butyryl citrate, urea, shellac, iopromide and polysorbate orsorbitol. Though they differ in functions (Scheller 2004), preferable excipients have better ability of facilitating anti-proliferative drugs to penetrate into the arterial wall. A series of studies have found that the combination of lipophilic paclitaxel and hydrophilic excipients is the most effective coating type since this match helps to enhance the paclitaxel bioavailability (Cho et al. 2006). Additionally, the peptide derivative, RGDS-OC8H17, has been shown to be able to bind selectively to integrin-αvβ3 in vascular endothelial cells thus increasing drug retention and release in tissues (Dong et al. 2009; Pallarola et al. 2014; Turner et al. 2018).
Hence in this study, we first chose pectin, a natural high-molecular hydrophilic polysaccharide as the excipient (Cortese et al. 2010); then successively designed two novel PCB-based coating solutions: pectin-paclitaxel and pectin-RGDS-OC8H17-paclitaxel (nanoparticle was used in the latter), and finally characterized them in terms of morphology and drug-releasing curve in followed in vitro experiments by comparing with each other and with the commercially available shellac excipient coating.
Materials and methods
Materials
The purchasing information of the materials used in this study is as follows. Eggplant-shaped bottles (50 mL), graduated cylinders (10 mL) and glass rods were purchased from Synthware Glass (China). Absolute ethyl alcohol (EtOH, CAS: 64-17-5), glycerol (CAS: 56-81-5), Chromatographic acetonitrile (CAS: 75-05-8), NaOH (CAS: 1310-73-2), NaHCO3 (CAS: 144-55-8) were purchased from Beijing Chemical Co. Ltd.. Paclitaxel were purchased from Sigma-Aldrich (USA). Pectin and bleach dewax shellac were purchased from Chuxiong Deersi shellac (China). All chemicals purchased were used as received. And RGDS-OC8H17 was synthesized in our lab.
Preparation of pectin-RGDS-OC8H17-paclitaxel coating solution.
The even coating solution was comprised of paclitaxel, pectin matrix and RGDS-OC8H17, wherein the mass ratio of pectin matrix to paclitaxel, pectin matrix to RGDS-OC8H17 and paclitaxel to RGDS-OC8H17 is 37.5: 1, 100: 1 and 2.7: 1, respectively. Initially, the mixtures of 1.9 g pectin, 72 μL glycerol and 19 mg RGDS-OC8 H17 were dissolved in 30 mL ultra-pure water by stirring in the oil bath at 40 °C. In parallel, pH of the mixtures was adjusted to 7–7.5 with 2 N NaOH solution, then a pectin colloidal dispersion system, which mixed with RGDS-OC8H17, was obtained. Paclitaxel solution were prepared in ethanol by adding 90 μL of the mixture solution into 100 μL of paclitaxel-ethanol solution (15 mg/mL). The ultra-pure water (400 μL) was then added dropwise into the same volume of paclitaxel solution to prepare pectin-RGDS-OC8H17-paclitaxel drug coating solution. In contrast, the pectin-paclitaxel drug coating solution in the control group does not contain RGD sequence peptide, and the bicarbonate solution is preferably selected to adjust the pH value, 5% sodium hydrogen carbonate solution is optimal.
Determination of drug-releasing curve
One purpose of this study was to measure the drug release curve via high performance liquid chromatography (HPLC). The in vitro trail of the drug release was carried out at a constant temperature of 37 °C and the release medium was de-ionized water (di-H2O). Initially, pectin-paclitaxel drug coating, shellac-paclitaxel drug coating and pectin-RGDS-OC8H17-paclitaxel drug coating were pipetted into six-well plate separately, and incubated at 37 °C for 4 days. Then, briefly, the drug, which coated on the six-well plate, were dissolved in 4 mL of di-H2O by oscillated the drug in the solvent at 300 rpm for 0 h, 2 h, 6 h, 12 h, 24 h, 36 h, 48 h, respectively, at 37 °C constant temperature. To optimize the quick-release curve, we increased the oscillation speed to 1200 rpm for 0 min, 10 min, 30 min, 60 min and drawn the cumulative release curve. Taken out release medium at each predetermined time point and replenish the same volume of deionized water.
Determination of residue paclitaxel
To determine the possible drugs losses during the experimental, we investigated the content of paclitaxel remained on the surface of balloon at the end of release time. After release tests, the six-well plate were incubated, followed by stirred and oscillated with 4 mL acetonitrile, until the paclitaxel in coating were completely dissolved. The residue of paclitaxel was measured using HPLC.
Characterization of drug coating solution
This study also proved that RGDS-OC8H17 added into the drug coating solution can improve the effect of drug delivery by scanning electron microscope (SEM) observation. The lyophilized pectin powder group and paclitaxel group were set as control groups, and the surface morphology and the nano-size of paclitaxel-RGDS-OC8H17 group were observed, as well as shellac-paclitaxel group, pectin-paclitaxel group and pectin-RGDS-OC8H17-paclitaxel group with different concentrations.
Preparation and characterization of lyophilized pectin powder
The pectin colloid without PGDS-OC8H17 was lyophilized after sonication. And the prepared naked-eye-visible powder was sprinkled on the surface of the silicon wafer for SEM imaging.
Preparation and characterization of paclitaxel powder
The paclitaxel powder was scattered directly on the surface of the silicon wafer for SEM imaging.
Preparation and characterization of paclitaxel-RGDS-OC8H17 drug coating solution
Briefly, paclitaxel-RGDS-OC8H17 mixtures were prepared by sonicated the mixture of 100 μL paclitaxel stock solutions (15 mg/mL) and 0.1 mg RGDS-OC8H17, and pipetted the mixture solution into watch glass. Followed by wrapped the watch glass with preservative film and pricked the tapes, and incubated at 37 °C for 4 days. Then, the paclitaxel-RGDS-OC8H17 drug coating solution was characterized using SEM.
Preparation and characterization of pectin-paclitaxel drug coating solution with different concentrations
Initially, the prepared pectin colloidal dispersion system without RGDS-OC8H17 (90 μL) were mingled with 100 μL paclitaxel-ethanol solution (15 mg/mL) and stored at – 4 °C refrigerator to obtained the mother liquor (1–9 × 10–3 mol/L). Then, the mother liquor was diluted by 100 times and 1000 times respectively to obtain homogeneously milky white liquid samples with drug concentration were about 1–9 × 10–5 mol/L and 1–9 × 10–6 mol/L. Followed by pipetted 10 μL of each sample into the silicon wafer, and these showed milky white sphere on the surface of silicon wafer due to the large surface tension. In parallel, placed a piece of filter paper on the watch glass, divided the area and record the information of samples on the silicon wafer, then put into three pieces of silicon wafer in turn, wrapped the watch glass with preservative films, pricked the tapes and placed the watch glass in a 37 °C incubator for 4 days to dry. The pectin-paclitaxel drug coating solution was characterized using SEM.
Preparation and characterization of shellac-paclitaxel drug coating solution with different concentrations
Initially, the mixture of 1.9 mg shellac and 72 μL glycerol were dissolved in 10 mL ultra-pure water by stirring in the oil bath at 40 °C and adjust pH value of the mixture to 7–8 with saturated NaHCO3 solution. Shellac-paclitaxel solution were prepared after the addition of paclitaxel-ethanol solution 100 μL (15 mg/mL) and stored at − 4 °C refrigerator. Then, the mother liquor was diluted by 100 times and 1000 times respectively to obtain homogeneously liquid samples with drug concentration were about 1–9 × 10–5 mol/L and 1–9 × 10–6 mol/L. Followed by pipetted 10 μL of each sample into the silicon wafer and placed a piece of filter paper on the watch glass, divided the area, record the information of samples on the silicon wafer, and put into three pieces of silicon wafer in turn. Followed by wrapped the watch glass with preservative films, pricked the tapes and place the watch glass in a 37 °C incubator for 4 days to dry. The shellac-paclitaxel drug coating solution was characterized using SEM.
Preparation and characterization of pectin-RGDS-OC8H17-paclitaxel drug coating solution with different concentrations
Firstly, the prepared pectin colloidal dispersion system with RGDS-OC8H17 (90 μL) were mingled with 100 μL paclitaxel-ethanol solution (15 mg/mL) and stored at − 4 °C refrigerator to obtained the mother liquor (1–9 × 10–3 mol/L). Then, the mother liquor was diluted by 100 times and 1000 times respectively to obtain evenly milky white liquid samples with drug concentration are about 1–9 × 10–5 mol/L and 1–9 × 10–6 mol/L. Followed by pipetted 10 μL of each sample into the silicon wafer and placed a piece of filter paper on the watch glass, divided the area, record the information of samples on the silicon wafer, and put into three pieces of silicon wafer in turn. Followed by wrapped the watch glass with preservative films, pricked the tapes and place the watch glass in a 37 °C incubator for 4 days to dry. The pectin-RGDS-OC8H17-paclitaxel drug coating solution was characterized using SEM.
Statistical analysis
The experiments were conducted nine times per group unless otherwise specified and were noted in every experiment. Quantitative values of data are represented as mean ± standard deviation (SD). Comparisons among groups were conducted by a one-way analysis of variance (ANOVA). The experimental groups were compared with the control groups by student t-test. And a value of P < 0.05 was considered statistically significant difference.
Result
Drug release from balloon
The drug releasing profile was determined in Fig. 1. And Fig. 1a shows the amount of shellac-paclitaxel coating released at different time point with 300 rpm. For shellac-paclitaxel coating, there was almost no drug released during the time span of 48 h. For pectin-paclitaxel coating, the percentage of paclitaxel released was more than 90% for up to 2 h, and almost 99% of paclitaxel were released during the time of 6 h. As for pectin-RGDS-OC8H17-paclitaxel coating, its releasing curve was similar with pectin-paclitaxel group, which shows a quickly releasing profile at the first time point (2 h) then released reposefully within 2–6 h and stayed stable until the end of experiments.
However, this coating was design to treat various periphery arterial diseases and prevent restenosis, and the major factor for the large losses of drug during the operation is the scour of blood stream. To better simulate the releasing of drug coating in the blood vessel, we optimized the releasing curve by increased oscillation speed to 1200 rpm for 0, 10, 30, 60 min (Fig. 1b).
For pectin-paclitaxel coating, more than 99% of paclitaxel were released in the initial 10 min and almost released no more for up to the end. As for pectin-RGDS-OC8H17-paclitaxel coating, its releasing curve was similar with pectin-paclitaxel group, which experienced a fast released during the first 10 min and seems kept still in the rest period.
These results fully proofed that the release of paclitaxel from pectin excipient cannot be changed by the incorporation of RGDS-peptide. What’s more, the addition of pectin can improve the ability of DCB releasing paclitaxel at the target lesion.
Drug residue of balloon
The total amount of paclitaxel loaded on the balloon is 2 mg. For shellac-paclitaxel coating, the drug retained on the surface of balloon is far more than pectin-paclitaxel coating (630.4 ± 2.1 μg and 15.8 ± 2.4 μg, respectively, P < 0.01). The amount of surface residue is pectin-RGDS-OC8H17-paclitaxel coating 18.6 ± 0.3 μg (P < 0.01) (Table 1).
Characterization of drug coating solution
Morphologic observation of drug coating
Figure 2 shows the images of Shellac-paclitaxel coating (Fig. 2a), pectin-paclitaxel coating (Fig. 2b) and pectin-RGDS-OC8H17-paclitaxel coating (Fig. 2c), they were fully coated on glass slide for direct observation. Both coatings were smooth, uniform and homogeneous on the glass slide surface, and pectin-RGDS-OC8H17-paclitaxel coating was better.
SEM characterization of freeze-dried powder
The overall dispersion of pectin freeze-dried powder sample was not uniform, and some of them were clustered together and rod-shaped pectin structure can also be seen in the field of view (Fig. 2d). Well-dispersed pectin should be globules in diameter of about 50 nm.
Globular paclitaxel (Fig. 2e), ranging in diameter from 0.8 to 3 μm, was adhered to each other and some of them exist surface gap, and the others were discoid and adhere irregularity to each other.
For paclitaxel-RGDS-OC8H17 mixtures (Fig. 2f), paclitaxel and RGDS-OC8H17 were sphere ranging in diameter from 300 to 700 nm and dispersed uniformly, a few adherence of them were occasionally seen. The whole visual field was foggy, which indicated that two substances have a certain adhesion after formed and the formation can be evenly coated.
SEM characterization of drug coating
These SEM images presented that with the decrease of drug concentration, the aggregation of reticular formed shellac decreased, but the interspace increased (Fig. 3a, d and g). And in 10−6 mol/L coating, images showed mostly scattered filaments with occasional spindles and white particles, and the particles could be paclitaxel. What is more, in 10–4 mol/L and 10–6 mol/L coating, globule paclitaxel were visible, 500 nm and 300–700 nm in diameter, respectively.
Figure 3b, e and h shows pectin-paclitaxel coating SEM images in different drug concentration. For 10–3 mol/L and 10–5 mol/L pectin-paclitaxel coating, most of them were crystalloid, and they were rod-shaped when the visual field was enlarged, ranging in diameter from 0.7–7 μm to 0.7–3 μm, respectively. As for 10–6 mol/L pectin-paclitaxel coating, they were more evenly dispersed, mostly in the form of scattered rod-like with the length of 0.7–2 μm, and the width, ranging from 100–200 nm, which were smaller than their high concentrations. Globule paclitaxel can be seen in the interlaced claviform pectin which is mainly between 300 and 900 nm in diameter, and more uniform interlaced rod-like compared with high concentrations. The whole visual field was foggy, which also indicated that two substances have a certain adhesion after mixed and the formation can be evenly coated.
The SEM images of pectin-RGDS-OC8H17-paclitaxel coating of different drug concentrations were shown in Fig. 3c, f and i. For 10–3 mol/L and 10–5 mol/L pectin-RGDS-OC8H17-paclitaxel coating, the morphological characteristics and diameter of them were similar with the drug coating without RGDS-OC8H17. On different coating concentrations of 10–5 mol/L and 10–6 mol/L, the SEM images showed more claviform pectin and the diameter of pectin were 0.7–2 μm in 10−6 mol/L coating and 0.7–3 μm in 10–5 mol/L. In the same way, we can found some globule paclitaxel in the interlaced pectin with diameter from 400–900 nm in 10–5 mol/L coating and 300–700 nm in 10–6 mol/L coating, respectively. As the concentration of the coating drug decreased, more claviform pectin was observed and the length was smaller, and globular paclitaxel were mixed more evenly with pectin. The gap between pectin and RGDS-OC8H17 and paclitaxel presents a fog-like shadow, indicating that there is a certain adhesion after three substances are mixed and can be evenly coated.
Discussion
In this study, we have demonstrated the feasibility of using pectin as the excipient to improve drug release efficiency and adding RGDS-OC8H17 peptide to achieve fixed-point release particularly compared with the performance of shellac excipients.
Pectin is a natural high-molecular polysaccharide, its main component being polygalacturonic acid linked by α-1,4 glycosidic bonds. As an alternative excipient for balloon coating, pectin is more affordable and attainable. It also has better biocompatibility, much safety, and complete biodegradability (Cortese et al. 2010). In previous studies, pectin was used as an ideal drug delivery vehicle or matrix for colon-specific drug delivery systems due to its strong hydrophilic property. It would disperse in the aqueous solution and then swell to form a colloidal dispersion system (Cortese et al. 2010).
Shellac is the excipient used for the drug-coated balloon currently commercially available. The resin portion of the shellac skeleton is composed of various sesquiteric acid and hydroxy fatty acid (Qi et al. 2016; Hong and Shu 2017). It was used as a coating material to adjust the disintegration performance of tablets, and to prevent the inner layer of the drug from moisture and degradation. But the significant critical problem is that shellac, which acts as an excipient, shows poor performance in a vital step of rapidly release.
RGDS-petide is the receptor ligands of glycoprotein IIb/IIIa (GP IIb/IIIa) on the platelet surface, and GP IIb/IIIa could be recognized by binding sites in RGDS, thus RGDS has a tropism towards platelets in thrombus (Varga-Szabo et al. 2008; Imura et al. 1992). Some experiments and clinical trials have shown that RGDS have high adhesion and bioactivity to osteoblasts and fibroblasts, and target marrow stroma cells, smooth muscle cells and endothelial cells (Zhu et al. 2000). It has been reported that RGDS is used to improve the targeting of thrombolytic drugs in animal experiments (Szemraj et al. 2011). Some studies have also explored the potential of RGDS in targeted thrombolytic therapy and tumor targeting carrier (Guan et al. 2020; Cui et al. 2015). And a kind of liposomes, which surface functionalised by a cyclic arginly-glycyl-aspartic acid (cRGD) peptide, have been designed for thrombosis target treatment (Chandarana et al. 2018). Besides, a peptide containing the Arg-Gly-Asp (RGD) sequence have the ability of selectively combined with integrin-αvβ3 which plays a crucial role in angiogenesis and metastasis and closely related to the invasion and metastasis of tumor cells, such as endothelial cells and malignant melanoma, breast cancer and advanced glioblastoma (Hood John and Cheresh David 2002; Ryo and Michiya 2002; Demircioglu and Hodivala-Dilke 2016). When they are in the condition of vascular proliferation, defective vessel and poor lymph drainage or recovery system, nano-particles will exosmose from bloodstream because the production of osmotic regulators (Dong et al. 2009). Nano-drugs that combine RGDS-peptides could accumulate in these cells either actively by target molecule or passively by enhanced permeability and retention (EPR) effects, increasing efficacy and reducing side effects (Pallarola et al. 2014; Fang et al. 2020).
As for in vitro release experiment of this study, we found that, in 300 rpm, the paclitaxel in pectin was released up to 99% at 6 h. However, in 1200 rpm, it took only 10 min to reach same releasing amount. In comparison, the paclitaxel in shellac was almost not released within 24 h, and most of them remained on the balloon surface. It indicates that pectin has ability of adjustable paclitaxel release in vitro and rapidly release under the circumstance of bloodstream.
By SEM image, we found that the concentration of pectin solutions could affects the degree of pectin decomposition, and then affects the dispersion of drugs and pectins. SEM image of pectin-RGDS-OC8H17-paclitaxel mixtures, the form of pectins were not changed obviously, but the thinner pectin-RGDS-OC8H17-paclitaxel solutions, the better dispersion, the less aggregation, and smaller rod-like pectins with scattered spherical RGDS-OC8H17-paclitaxel compounds (400–900 nm). The globular RGDS-OC8H17-paclitaxel nanoparticles were well mixed and interlaced with pectin, and the gap between them showed a cloud shadow, which indicated that pectin-RGDS-OC8H17-paclitaxel mixture have adhesion and can be evenly coated. As for shellac-paclitaxel mixture, in most conditions, paclitaxel is wrapped in the grid of shellac, making it hard to release.
A possible explanation is that RGDS-OC8H17 compounds has hydrophilic exposed amino groups and hydrophobic carbon chain, acting as link between hydrophilic pectins and hydrophobic paclitaxel to promote the well mixture of pectin, RGDS-OC8H17 and paclitaxel. Moreover, since RGDS could locate vascular endothelial cells and target GP IIb/IIIa, it is reasonable to believe that when pectin-RGDS-OC8H17-paclitaxel coating is released from balloon, RGDS-OC8H17 peptide could guide paclitaxel convey to target lesion, increasing the ability of location and reducing the adverse reactions caused by drug losses in bloodstream.
During the endovascular operation, only 5 ~ 20% of drug is transferred into the vessel wall and 10% remain on the balloon (Speck et al. 2016). To increase the potency, several combinations of drug-coatings have been found, such as sirolimus nanoloposomal formulation (Nanolimus), polydimethylsiloxane (PDMS), everolimus and paclitaxel / resveratrol (Speck et al. 2018; Ong et al. 2016; Yamamoto et al. 2018; Ang et al. 2020). Nevertheless, the performance of pectin-RGDS-OC8H17-paclitaxel coating is specific and has not been reported. Its ability of rapidly release in vivo and targeted transport drug to diseased vessel segment accurately would be further proved by in vivo experiments.
Conclusion
The pectin-RGDS-OC8H17-paclitaxel-coated balloon developed in this study exhibited the advantages of faster drug release and more accurate endothelial cells location compared to commercially available DCB with shellac excipients in this study. Thus, the study demonstrated that pectin could act as a next generation excipient to effectively release drug and RGDS could play a role of a locator to accurately connect paclitaxel to target lesion.
References
Ang HY, Xiong GM, Chaw SY, Phua JL, Ng JCK, Wong PEH, Venkatraman S, Chong TT, Huang Y (2020) Adventitial injection delivery of nano-encapsulated sirolimus (Nanolimus) to injury-induced porcine femoral vessels to reduce luminal restenosis. J Control Release 319:15–24
Chandarana M, Curtis A, Hoskins C (2018) The use of nanotechnology in cardiovascular disease. Appl Nanosci 8:1607–1619
Cho HJ, Kim TY, Cho HJ et al (2006) The effect of stem cell mobilization by granulocyte-colony stimulating factor on neointimal hyperplasia and endothelial healing after vascular injury with bare-metal versus paclitaxel-eluting stents. J Am Coll Cardiol 48(2):366–374
Cortese B, Micheli A, Picchi A et al (2010) Paclitaxel-coated balloon versus drug-eluting stent during PCI of small coronary vessels, a prospective randomised clinical trial The PICCOLETO study. Heart 96(16):1291–1296
Criqui MH, Browner D, Fronek A, Klauber MR, Coughlin SS, Barrett-Connor E, Gabriel S (1989) Peripheral arterial disease in large vessels is epidemiologically distinct from small vessel disease. An analysis of risk factors. Am J Epidemiol 129:1110–1119
Criqui Michael H (2015) Aboyans Victor, Epidemiology of peripheral artery disease. Circ Res 116:1509–1526
Cui C, Wang Y, Zhao W, Yang K, Jiang X, Li S, Zhao M, Song Y, Peng S (2015) RGDS covalently surfaced nanodiamond as a tumor targeting carrier of VEGF-siRNA: synthesis, characterization and bioassay. J Mater Chem B 3(48):9260–9268
Demircioglu F, Hodivala-Dilke K (2016) αvβ3 Integrin and tumour blood vessels-learning from the past to shape the future. Curr Opin Cell Biol 42:121–127
Dong X, Wei X, Yi W, Gu C, Kang X, Liu Y, Li Q, Yi D (2009) RGD-modified acellular bovine pericardium as a bioprosthetic scaffold for tissue engineering. J Mater Sci Mater Med 20(11):2327–2336
Fang J, Islam W, Maeda H (2020) Exploiting the dynamics of the EPR effect and strategies to improve the therapeutic effects of nanomedicines by using EPR effect enhancers. Adv Drug Deliv Rev 157:142–160
Gerhard-Herman Marie D, Gornik Heather L, Coletta B et al (2017) 2016 AHA/ACC guideline on the management of patients with lower extremity peripheral artery disease: executive summary: a report of the american college of cardiology/american heart association task force on clinical practice guidelines. Circulation 135:e686–e725
Gongora CA, Shibuya M, Wessler JD, McGregor J, Tellez A, Cheng YP, Conditt GB et al (2015) Impact of paclitaxel dose on tissue pharmacokinetics and vascular healing a comparative drug-coated balloon study in the familial hypercholesterolemic swine model of superficial femoral in-stent restenosis. JACC Cardiovasc Interv 8(8):1115–1123
Guan L, Wang C, Yan X, Liu L, Li Y, Mu Y (2020) A thrombolytic therapy using diagnostic ultrasound combined with RGDS-targeted microbubbles and urokinase in a rabbit model. Sci Rep 10(1):12511
Heilmann T, Richter C, Noack H, Post S, Mahnkopf D, Mittag A, Thiele H et al (2010) Drug release profiles of different drug-coated balloon platforms. Eur Cardiol Rev 6(4):40–44
Hong WT, Shu BQ et al (2017) Advance in drug-coated balloon research. Chin Circ J 32(11):1134–1136
Hood John D, Cheresh David A (2002) Role of integrins in cell invasion and migration. Nat Rev Cancer 2:91–100
Imura Y, Stassen JM, Bunting S, Stockmans F, Collen D (1992) Antithrombotic properties of L-cysteine, N-(mercaptoacetyl)-D-Tyr-Arg-Gly-Asp-sulfoxide (G4120) in a hamster platelet-rich femoral vein thrombosis model. Blood 80(5):1247–1253
Ong PJL, Kubo T, Watson TJ, Seah ASP, Wong PEH, Akasaka T (2016) Angiographic, optical coherence tomography and histology findings from combination of a drug-coated balloon with an everolimus-eluting stent in a porcine model. Int J Cardiol 223:665–668
Pallarola D, Bochen A, Boehm H, Rechenmacher F, Sobahi TR, Spatz JP, Kessler H (2014) Interface immobilization chemistry of cRGD-based peptides regulates integrin mediated cell adhesion. Adv Funct Mater 24(7):943–956
Qi ZX, Jing ZS, Jin L et al (2016) Pharmacokinetic study of peripheral drug-coated balloon in swines. China Med Device Inform 22(10):1–3
Ryo H, Michiya K, Toshihiko M et al (2002) Expression of integrin alphaVbeta3 in pancreatic carcinoma: relation to MMP-2 activation and lymph node metastasis. Pancreas 25:e30-35
Sarode K, Spelber DA, Bhatt DL, Mohammad A, Prasad A, Brilakis ES, Banerjee S (2014) Drug delivering technology for endovascular management of infrainguinal peripheral artery disease. JACC Cardiovasc Interv 7(8):827–839
Scheinert D, Duda S, Zeller T, Krankenberg H, Ricke J, Bosiers M, Tepe G et al (2014) The LEVANT I (Lutonix paclitaxel-coated balloon for the prevention of femoropopliteal restenosis) trial for femoropopliteal revascularization first-in-human randomized trial of low-dose drug-coated balloon versus uncoated balloon angioplasty. JACC Cardiovasc Interv 7(1):10–19
Scheller B (2004) Paclitaxel balloon coating, a novel method for prevention and therapy of restenosis. Circulation 110(7):810–814
Speck U, Stolzenburg N, Peters D, Scheller B (2016) How does a drug-coated balloon work? overview of coating techniques and their impact. J Cardiovasc Surg (Torino) 57(1):3–11
Speck U, Häckel A, Schellenberger E, Kamann S, Löchel M, Clever YP, Peters D, Scheller B, Trog S, Bettink S (2018) Drug distribution and basic pharmacology of paclitaxel/resveratrol-coated balloon catheters. Cardiovasc Intervent Radiol 41(10):1599–1610. https://doi.org/10.1007/s00270-018-2018-9 (Epub 2018 Jul 2)
Sur JP, Casserly IP, Gurm HS (2011) Balloon and stent technology. In: Casserly IP, Sachar R, Yadav JS (eds) Practical peripheral vascular intervention, 2nd edn. LWW, Philadelphia
Szemraj J, Zakrzeska A, Brown G, Stankiewicz A, Gromotowicz A, Grędziński T, Chabielska E (2011) New derivative of staphylokinase SAK-RGD-K2-Hirul exerts thrombolytic effects in the arterial thrombosis model in rats. Pharmacol Rep 63(5):1169–1179
Turner E, Erwin M, Atigh M et al (2018) In vitro and in vivo assessment of keratose as a novel excipient of paclitaxel coated balloons. Front Pharmacol 9:808
Varga-Szabo D, Pleines I, Nieswandt B (2008) Cell adhesion mechanisms in platelets. Arterioscler Thromb Vasc Biol 28(3):403–412. https://doi.org/10.1161/ATVBAHA.107.150474 (Epub 2008 Jan 3 PMID: 18174460)
Yamamoto E, Watanabe S, Bao B, Watanabe H, Nakatsuma K, Izuhara M, Ono K, Nakazawa G, Kimura T, Saito N (2018) Preclinical evaluation of a paclitaxel-incorporated nanoparticle-coated balloon in rabbit and porcine models. Cardiovasc Revasc Med 19(4):433–437
Zhu C, Bao G, Wang N (2000) Cell mechanics: mechanical response, cell adhesion, and molecular deformation. Annu Rev Biomed Eng 2:189–226
Funding
This study was supported by the Beijing Municipal Colleges and Universities High Level Talents Introduction and Cultivate Project-Beijing Great Wall Scholar program (CIT&TCD 20180332), Scientific Research Common Program of Beijing Municipal Commission of Education (KM201810025011).
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Wang, P., Gui, L., Wang, Y. et al. In vitro evaluation of nanoparticle drug-coated balloons: a pectin-RGDS-OC8H17-paclitaxel solution. Appl Nanosci 11, 1339–1347 (2021). https://doi.org/10.1007/s13204-021-01736-4
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DOI: https://doi.org/10.1007/s13204-021-01736-4